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Application of flavones extract of ficus virens leaves

A technology of leaf flavonoids and extracts, applied in the field of medicine, can solve the problem of no definite and effective drugs and treatment methods, and achieve the effects of good microglial inflammatory activation and inhibition of microglial inflammatory activation

Inactive Publication Date: 2018-10-16
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no effective drugs and treatments for these diseases

Method used

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  • Application of flavones extract of ficus virens leaves
  • Application of flavones extract of ficus virens leaves
  • Application of flavones extract of ficus virens leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 prepares the flavonoids purified product of the leaves of Jue chinensis

[0030] 1. Materials

[0031] Juice leaves (collected from the campus of Chongqing Medical University); standard rutin was purchased from China Institute for the Control of Pharmaceutical and Biological Products; AB-8 macroporous adsorption resin was purchased from Beijing Suolai Biotechnology Co., Ltd.; BV-2 microglial cells Sourced from the Department of Molecular Genetics, Chongqing Medical University; DMEM medium and fetal bovine serum were purchased from GIBCO, USA; CCK-8 kit was purchased from Beyond Biotechnology Research Institute; Nitric Oxide (NO) assay kit was purchased from Nanjing Jiancheng Institute of Bioengineering; mouse interleukin-1β (IL-1β) ELISA kit was purchased from Xinbosheng Biotechnology Co., Ltd.; LPS and dimethyl sulfoxide (Dimethyl sulfoxide, DMSO) were purchased from sigma; all other reagents were Analytical grade was purchased from a commercial reagent c...

Embodiment 2

[0053] Anti-inflammatory activity assay of embodiment 2 flavonoids purification

[0054] 1. Method

[0055] 1. Establishment of BV-2 cell LPS inflammatory activation model

[0056] BV-2 cells were incubated in DMEM medium containing 10% inactivated FBS, 100 U / mL penicillin, and 100 mg / L streptomycin at 37°C, 5% CO 2 Routine culture in the incubator, when the cells reached the logarithmic growth phase, they were digested and passaged with 0.125% trypsin. After the cells were inoculated and cultured for 16 hours to adhere to the wall, the corresponding drug treatment was carried out. After LPS was dissolved in DMSO, it was diluted with DMEM medium to treat the cells; the purified flavone was diluted with DMEM medium to treat the cells with the corresponding concentration. According to the results of the cell viability test, the best intervention concentration of LPS and purified flavonoids is selected.

[0057] 2. Determination of cell viability (CCK-8 method)

[0058] BV-2...

Embodiment 3

[0079] Example 3 Study on the protection of mouse nerve cell LPS inflammatory injury by the extract

[0080] Our studies on cells cultured in vitro showed that the extract of flavonoids from the leaves of Jue quinquefolium could protect PC12 cells from the damage caused by the activation of BV2 microglial cells. In order to further study the inhibitory effect of the flavonoids extract from the leaves of the quince tree on the activation of microglia and whether it can protect the nerve cells from inflammatory injury, we established a C57BL / 6 mouse model of LPS inflammatory injury, by measuring the flavonoids extract from the leaves of the pine tree The effect of drug administration on the number of activated microglial cells in the substantia nigra of model mice, and the effect on the number of dopaminergic neurons in the substantia nigra of the model mice, to study the activation of microglial cells in the substantia nigra damage to nerve cells. The result is as follows:

...

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Abstract

The invention discloses application of flavones extract of ficus virens leaves in preparing a drug for inhibiting microglial cell inflammatory activity, and application of flavones extract of ficus virens leaves in preparing a drug for preventing or treating neurodegenerative diseases as well as application of flavones extract of ficus virens leaves in a healthcare product or food. The flavones extract of ficus virens leaves is found to have good effect in inhibiting microglial cell inflammatory activity, has a good application prospect in possibly preventing or treating neurodegenerative diseases such as Parkinson's disease and alzheimer's disease, further provides a basis for developing a safe and effective drug source, provides basis for effectively utilizing rich ficus virens resourcesin a Chongqing area, and has important scientific and social meanings.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of a flavonoid extract from the leaves of the Jujube tree. Background technique [0002] Yellow Jue tree (also known as Huanggeshu, Daye Banyan, and Ponytail Banyan) is a tall deciduous tree with a tall crown. It is one of the commonly used shade trees and street trees; it is native to South China and Southwest China, and has strong environmental tolerance. There are many distributions in Sichuan, Hubei and other places. In Chongqing, resources are abundant, widely distributed from towns, villages, riversides, ditches, fields, temples, schools, parks, roadsides to rock crevices, and are the city tree of Chongqing. It is recorded in "National Compendium of Chinese Herbal Medicine" and "Chinese Materia Medica" that the roots and leaves of the Jue tree can be used as medicine. In experiential medicine, the root of the Jueper tree is often used for expelling wind and dampnes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60A61P25/28A61P25/16A61P25/00A23L33/105A61K127/00
CPCA23V2002/00A61K36/60A23L33/105A61P25/00A61P25/16A61P25/28A23V2200/322
Inventor 白群华陈玲唐勇
Owner CHONGQING MEDICAL UNIVERSITY
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