Compositions and methods for preventing and/or treating vitamin b12 deficiency

Abstract

A method for preventing and / or treating vitamin B12 deficiency is taught. The method comprises administering a composition comprising pseudovitamin B12 producing bacteria, optionally in conjunction with mucin-degrading and / or propionate-producing bacteria, to a subject in need thereof. The method is particularly suitable for administration to subjects suffering from vitamin B12 deficiency due to metformintreatment fortype-2 diabetes, and to subjects having undergone bariatric surgery.

Description

technical field

[0001] The present invention relates to the fields of vitamin B12 production and prevention and / or treatment of vitamin B12 deficiency, intestinal microbiota, intestinal mucosal barrier, and pharmaceutical, food or feed compositions or probiotic compositions comprising Pseudovitamin B12-producing bacteria E. hallii and / or I. butyriciproducens and optionally one or more propionate-producing bacteria and / or mucin Degrading bacteria such as A. muciniphila. The present invention also provides Eubacterium hallii and / or Enteromonas butyrinigenes and a compound comprising Eubacterium hallii and / or Enteromonas butyrinogen and optionally propionate-producing bacteria and / or mucin Compositions of degrading bacteria (e.g. Ekkermans muciniphila) for the following uses and methods: 1) for preventing and / or treating vitamin B12 deficiency in an individual, 2) for promoting and / or increasing vitamin B12 deficiency in an individual production of pseudovitamin B12 in the gut,...

Examples

Embodiment Construction

[0051] The present inventors have surprisingly found that a composition comprising Eubacterium hallii and / or Enteromonas butyrategenes and an acceptable carrier can be used for the treatment, prevention and / or Alleviate vitamin B12 deficiency. The present inventors have found that Eubacterium hallii and Enterococcus butyrategenes have the ability to produce pseudovitamin B12.

[0052] Without being bound by any theory, it is believed that Eubacterium hallii and Enterococcus butyrategenes produce and secrete pseudovitamin B12 in situ (ie, in the gut of an individual). The pseudovitamin B12 can then be immediately taken up by the host's intestinal cells, where it can be used directly as a cofactor for vitamin B12-dependent enzymes, or it can be converted to a different form of vitamin B12 for use in vitamin B12-dependent enzymes cofactors.

[0053] Pseudovitamin B12 has been shown to act as a cofactor for three vitamin B12-dependent enzymes MetH, EutBC and PduCDE in Salmonella...

technical field

[0001] The present invention relates to the fields of vitamin B12 production and prevention and / or treatment of vitamin B12 deficiency, intestinal microbiota, intestinal mucosal barrier, and pharmaceutical, food or feed compositions or probiotic compositions comprising Pseudovitamin B12-producing bacteria E. hallii and / or I. butyriciproducens and optionally one or more propionate-producing bacteria and / or mucin Degrading bacteria such as A. muciniphila. The present invention also provides Eubacterium hallii and / or Enteromonas butyrinigenes and a compound comprising Eubacterium hallii and / or Enteromonas butyrinogen and optionally propionate-producing bacteria and / or mucin Compositions of degrading bacteria (e.g. Ekkermans muciniphila) for the following uses and methods: 1) for preventing and / or treating vitamin B12 deficiency in an individual, 2) for promoting and / or increasing vitamin B12 deficiency in an individual production of pseudovitamin B12 in the gut,...

Examples

Embodiment Construction

[0051] The present inventors have surprisingly found that a composition comprising Eubacterium hallii and / or Enteromonas butyrategenes and an acceptable carrier can be used for the treatment, prevention and / or Alleviate vitamin B12 deficiency. The present inventors have found that Eubacterium hallii and Enterococcus butyrategenes have the ability to produce pseudovitamin B12.

[0052] Without being bound by any theory, it is believed that Eubacterium hallii and Enterococcus butyrategenes produce and secrete pseudovitamin B12 in situ (ie, in the gut of an individual). The pseudovitamin B12 can then be immediately taken up by the host's intestinal cells, where it can be used directly as a cofactor for vitamin B12-dependent enzymes, or it can be converted to a different form of vitamin B12 for use in vitamin B12-dependent enzymes cofactors.

[0053] Pseudovitamin B12 has been shown to act as a cofactor for three vitamin B12-dependent enzymes MetH, EutBC and PduCDE in Salmonella...

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