Large-scale entomopathogenic nematode production method and entomopathogenic nematode

Abstract

The invention belongs to the production field of entomopathogenic nematodes and relates to a large-scale entomopathogenic nematode production method and entomopathogenic nematodes. The production method comprises the steps that the entomopathogenic nematodes in an infection period are inoculated onto greater wax moths for infection, and the entomopathogenic nematodes are collected to prepare an entomopathogenic nematode suspension after the greater wax moths die; separated entomopathogenic nematodes are utilized to infect greater wax moths, then the entomopathogenic nematodes are separated from died greater wax moth bodies for single bacterium culture, and the entomopathogenic nematodes cultured based single bacteria are separated for production and propagation; the entomopathogenic nematodes in the infection period are utilized to infect living greater wax moth bodies, then died greater wax moth hemolymphs are collected, and a symbiotic bacterium solution for production is obtained through culture, authentication, adaption, fermentation and symbiotic bacterium propagation; the obtained symbiotic bacterium solution is firstly inoculated to breeding bags of a shared culture medium,and then the obtained entomopathogenic nematodes are inoculated into the breeding bags of the shared culture medium for culture. The large-scale entomopathogenic nematode production method is convenient to operate, capable of making product quality good and higher in yield and efficiency and makes factory-like large-scale breeding easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of production of entomopathogenic nematodes, and in particular relates to a large-scale production method of entomopathogenic nematodes and entomopathogenic nematodes. Background technique [0002] As a biological control factor, entomopathogenic nematodes have been studied for nearly 80 years at home and abroad. This is not only because it has many advantages, such as a wide range of hosts, active search ability, special control effect on soil-dwelling and borer pests, safe for humans and animals, will not pollute the environment, and will not produce resistance, etc. Moreover, it is not restricted by environmental protection regulations in many countries. It is easy to use, has a fast insecticidal speed, and can be mixed with chemical insecticides and has been widely used in the prevention and control of pests such as agriculture, forestry and pasture. [0003] Because this type of nematode has the dual c...

AI Technical Summary

Problems solved by technology

The basis for the application of entomopathogenic nematodes is the ability to culture them in large quantities at low cost, but the current method for nematode culture is solid culture, which consumes a lot of labor and is not conducive to the large-scale application of nematodes

Compared with solid culture, nematode liquid culture technology has low labor consumption in large-scale production, but it is easy to cause pollution and is difficult to control