Large-scale entomopathogenic nematode production method and entomopathogenic nematode

An entomopathogenic nematode technology and a production method, which are applied to the large-scale production method of entomopathogenic nematodes and the field of entomopathogenic nematodes, can solve the problems of unfavorable large-scale promotion and application of nematodes, large labor consumption, low labor consumption, etc. Technical difficulty, easy operation, and the effect of ensuring product quality

Inactive Publication Date: 2018-11-02
吉林省怡科农业生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The basis for the application of entomopathogenic nematodes is the ability to culture them in large quantities at low cost, but the current method for nematode culture is solid culture, which consumes a lot of labor and is not co

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0058] A method for large-scale production of entomopathogenic nematodes, comprising:

[0059] 1. Day 1 (infection of host insects)

[0060] Lay a layer of filter paper on the bottom of the petri dish, then place the mature larvae of the greater mellonella mellonella on the filter paper of the petri dish, add the nematode suspension, and place the petri dish in an incubator at 22-25°C for 24 hours.

[0061] 2. Day 2 (isolation of symbiotic bacteria)

[0062] After sterilizing the infected G. mellonella larvae with alcohol for 5-10 minutes, collect the G. mellonella hemolymph, spread the hemolymph on the surface of the culture medium, and culture it at 23-25°C for 3-4 days.

[0063] 3. Day 5 or Day 6 (identification and transfer of symbiotic bacteria)

[0064] After 3 to 4 days of culture, pick a single bacterial colony and check it under a microscope (it is rod-shaped), then transfer it, and continue to cultivate it on the slant bacterial breeding medium. 4 days.

[0065] ...

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Abstract

The invention belongs to the production field of entomopathogenic nematodes and relates to a large-scale entomopathogenic nematode production method and entomopathogenic nematodes. The production method comprises the steps that the entomopathogenic nematodes in an infection period are inoculated onto greater wax moths for infection, and the entomopathogenic nematodes are collected to prepare an entomopathogenic nematode suspension after the greater wax moths die; separated entomopathogenic nematodes are utilized to infect greater wax moths, then the entomopathogenic nematodes are separated from died greater wax moth bodies for single bacterium culture, and the entomopathogenic nematodes cultured based single bacteria are separated for production and propagation; the entomopathogenic nematodes in the infection period are utilized to infect living greater wax moth bodies, then died greater wax moth hemolymphs are collected, and a symbiotic bacterium solution for production is obtained through culture, authentication, adaption, fermentation and symbiotic bacterium propagation; the obtained symbiotic bacterium solution is firstly inoculated to breeding bags of a shared culture medium,and then the obtained entomopathogenic nematodes are inoculated into the breeding bags of the shared culture medium for culture. The large-scale entomopathogenic nematode production method is convenient to operate, capable of making product quality good and higher in yield and efficiency and makes factory-like large-scale breeding easy to achieve.

Description

technical field [0001] The invention belongs to the technical field of production of entomopathogenic nematodes, and in particular relates to a large-scale production method of entomopathogenic nematodes and entomopathogenic nematodes. Background technique [0002] As a biological control factor, entomopathogenic nematodes have been studied for nearly 80 years at home and abroad. This is not only because it has many advantages, such as a wide range of hosts, active search ability, special control effect on soil-dwelling and borer pests, safe for humans and animals, will not pollute the environment, and will not produce resistance, etc. Moreover, it is not restricted by environmental protection regulations in many countries. It is easy to use, has a fast insecticidal speed, and can be mixed with chemical insecticides and has been widely used in the prevention and control of pests such as agriculture, forestry and pasture. [0003] Because this type of nematode has the dual c...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李建平李茂海张金花刘金文田成丽滕占伟腾继深刘光宇王春久
Owner 吉林省怡科农业生物科技有限公司
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