Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bis(4-pyridine)cresol purple sultones as well as preparation method and application method thereof

A technology of violet sultone and violet sulfonic acid, which is applied in the field of albumin detection, can solve the problems of high detection value of albumin, increased light absorption value, inconvenient use, etc., and achieves simple operation, accurate detection results, and increased detection sensitivity. Effect

Inactive Publication Date: 2018-11-02
SHANDONG ZHUSHI PHARMA GRP CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this has caused the increase in the absorbance value in the detection of clinical serum, and the detected value of albumin is relatively higher than the true value, which has brought inconvenience to people's use.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bis(4-pyridine)cresol purple sultones as well as preparation method and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0022] A kind of two (4-pyridine) cresol purple sultone, its structural formula is

[0023] The preparation method of described two (4-pyridine) cresol purple sultone, concrete steps are as follows:

[0024] Step 1, add 1080mg compound 1-bromocresol purple (1eq, 2mmol), 1.542g potassium phosphate (3.6eq, 7.2mmol), 327mg tetrakis (triphenylphosphine) palladium (0.14eq, 0.28 mmol) and 1.07mg 4-pyridine boronic acid alcohol ester (2.6eq, 5.2mmol), then add 20ml N,N-dimethylformamide and 4ml water, heat to 100 degrees Celsius under the protection of argon, and carry out TLC to monitor the reaction process And the volume ratio of petroleum ether and ethyl acetate in the developer is 5:1, and the reaction is stopped after 6 hours to obtain the first reaction solution;

[0025] Step 2: The first reaction solution was concentrated and evaporated to dry...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bis(4-pyridine)cresol purple sultones as well as a preparation method and an application method thereof. The structural formula is shown in the description. The structure of the compound is to replace the bromine with 4-pyridine group on the basis of a bromcresol purple nuclear parent, so that a detection function of bromcresol purple sultones can be kept. The 4-pyridine group is used for substituting the bromine atoms to design and synthesize bis(4-pyridine)cresol purple sultones, so that bis(4-pyridine)cresol purple sultones has the advantage in detecting the contentof albumin in serum as follows: firstly, the poor water solubility of the bromcresol purple compound is changed, the water solubility of the compound is improved, and the detection sensitivity is improved; and secondly, bis(4-pyridine)cresol purple sultones contains no bromine atoms, so that the non-specific hydrophobic combination between the bromine atoms and various proteins can be reduced, andbis(4-pyridine) cresol purple sultones has better specificity for combining the albumin than that of the dye bromcresol purple compound, and can be used for detecting the content of the albumin.

Description

technical field [0001] The invention relates to the field of albumin detection, in particular to bis(4-pyridine)cresol violin sultone. Background technique [0002] The bromocresol purple method (BCP method) is a method for determining the content of albumin, which was mainly established in the 1970s. The principle of the experiment is that in the presence of Brij-35 in the buffer solution of pH 5.2, bromocresol purple (BCP) can combine with albumin to form a green complex, and the absorbance at a wavelength of 603nm is proportional to the concentration of albumin. Proportional, compared with the same treated albumin standard, the albumin content can be obtained. [0003] The dye method is used to determine the content of albumin in serum, which is easy to operate and is currently used by most clinical laboratories. However, there are many kinds of proteins in human serum, and the composition is extremely complex. Bromocresol green and bromocresol violet, two similar dyes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D411/14G01N21/33
CPCC07D411/14G01N21/33
Inventor 朱坤福祝蕾薛飞
Owner SHANDONG ZHUSHI PHARMA GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com