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Method for increasing yield and improving starch properties by using hydrogen ion pyrophosphorylase

A technology of phosphorylase and ion coke, which is applied in the field of plant biology, can solve problems such as difficulty in finding, difficulty in meeting the urgent needs of the starch processing industry, and long time consumption.

Active Publication Date: 2021-08-27
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relying on traditional breeding to change the quality of starch takes a long time, requires a lot of manpower and material resources, and is difficult to meet the urgent needs of the starch processing industry
[0006] Those skilled in the art have tried to regulate the composition and content of starch in plants by regulating the main genes of starch synthesis. However, there are many proteins related to starch synthesis, and it is still difficult to find really useful genes.

Method used

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  • Method for increasing yield and improving starch properties by using hydrogen ion pyrophosphorylase
  • Method for increasing yield and improving starch properties by using hydrogen ion pyrophosphorylase
  • Method for increasing yield and improving starch properties by using hydrogen ion pyrophosphorylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1, IBAVP1 transgenic sweet potato and production

[0102] In 2015, the inventors ported sweet potato to the Shanghai Wulian field, and observed the root type of the transgenic plants after harvest and the weight was measured. The results show that in the field environment, the roots of genetically modified sweet potatoes are greater than wild type. figure 1 A).

[0103] According to the statistical block root weight data, the weight of the transgenic sweet potato is significantly increased relative to the wild type. figure 1 B).

Embodiment 2

[0104] Example 2, Sweet potato IBAVP1 increases the size of the stem amount

[0105] The inventors have harvested wild-type and IBAVP1 transgenic sweet potato roots from the field, and analyzes the content of starch.

[0106] The results show that the starch content of the transgenic strain is significantly higher than the wild type. figure 2 ), An increase of approximately 20.07 to 31.91%.

Embodiment 3

[0107] Example 3, Sweet potato IBAVP1 increases the soluble sugar content in the root

[0108] The inventors extracted and analyzed changes in soluble sugar in wild type and genetically modified plants.

[0109] The results showed that glucose, fructose and sucrose content were significantly improved relative to the wild type content, and increased from 19.03 to 76.17%, 15.17 ~ 40.34%, 28.15 ~ 54.56% respectively. image 3 ).

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Abstract

The invention relates to a method for increasing yield and improving starch properties by using hydrogen ion pyrophosphorylase. It was revealed that hydrogen ion pyrophosphorylase (AVP1) from tuber plants has the effect of increasing the yield of tuber plants and improving the properties of starch in tuber roots of tuber plants. The invention can be applied to the variety improvement of potato plants.

Description

Technical field [0001] The present invention relates to the field of plant biology, and more particularly, the present invention relates to a method of improving production and modified starch properties using a hydrogen ionic acid. Background technique [0002] The potato crops represented by sweet potato have ultra-high light, heat, water utilization efficiency and unit area biomass, high starch content, and is suitable for the characteristics of the drought thin land without competing with food. It is currently the most Development of energy crops for development. [0003] Sweet potato, sweet potato, sweet potato species, for sputum herb. Sweet potato plants can be divided into roots, stems, leaves, flowers, fruits, seeds and other parts. Sweet potato is an important food crop and an important raw material in the food processing industry, rich in nutrition. Therefore, the optimization of sweet potato varieties and the study of cultivation and regenerative techniques are of gre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N9/14
CPCC12N9/14C12N15/8205C12N15/8245C12Q1/6895C12Q2600/13C12Q2600/158C12Y306/01001Y02A40/146
Inventor 张鹏范维娟吴银亮
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI