A preparation method of composite nanofiber membrane, composite nanofiber membrane and its application in enzyme immobilization and immobilized enzyme

A technology of composite nanofibers and nanofibers, applied in the direction of immobilized enzymes, immobilized on/in organic carriers, biochemical equipment and methods, etc., can solve problems affecting enzyme immobilization efficiency, unfavorable film formation, etc., and achieve improved Effect of Surface Area Ratio and Adsorption Effect, Multiple Adsorption Sites, Good Enzyme Loading Capacity

Active Publication Date: 2020-09-18
ANHUI POLYTECHNIC UNIV
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Problems solved by technology

[0004] Polypeptide nanofibers, as a new nanomaterial, usually have a diameter of only a few nanometers, have obvious nano-effects, have good adsorption properties and good biocompatibility, but polypeptide nanofibers are usually only a few nanometers in length, which is not conducive to film formation. , it is difficult to simply use polypeptide nanofibers to prepare membranes for enzyme immobilization, which will affect the efficiency of enzyme immobilization

Method used

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  • A preparation method of composite nanofiber membrane, composite nanofiber membrane and its application in enzyme immobilization and immobilized enzyme
  • A preparation method of composite nanofiber membrane, composite nanofiber membrane and its application in enzyme immobilization and immobilized enzyme
  • A preparation method of composite nanofiber membrane, composite nanofiber membrane and its application in enzyme immobilization and immobilized enzyme

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Embodiment Construction

[0029] Embodiments of the present invention provide a method for preparing a composite nanofiber membrane comprising polypeptide nanofibers, which includes the following steps:

[0030] (1) 130g terminal amino acid is negatively charged Ac-A 6 D-OH polypeptide and 135g of Ac-A whose terminal amino acid is positively charged 6 K-NH 2 Polypeptides, preferably according to the molar ratio of 1:1, are dissolved in trifluoroethanol to form a 1mg / mL solution to obtain a polypeptide mixture, which is stored at -20°C for later use. During implementation, the polypeptide mixture is diluted to 50ng.uL -1 , mixed with ethanol 4:1, cultured at room temperature for 6-10 days, preferably 8 days, to obtain a mixed solution containing polypeptide nanofibers for use, wherein the order of magnitude of the diameter of the polypeptide nanofibers is ten nm;

[0031] (2) With 3.6g of azobisisobutyronitrile as an initiator, weigh 150g of glycidyl methacrylate and 150g of methyl acrylate in 296.4g ...

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Abstract

The invention belongs to the field of nanofiber membranes, and particularly relates to a preparation method of a composite nanofiber membrane, the composite nanofiber membrane, application of the composite nanofiber membrane to enzyme immobilization and an immobilized enzyme. An electrostatic spinning solution containing chitosan is adopted for preparing the nanofiber membrane, and the membrane has a good film-forming property and an enzyme-immobilizing function; meanwhile, polypeptide nanofibers are adopted and loaded onto the membrane, in this way, the property of the membrane is further improved, and the enzyme-immobilizing property of the membrane is improved.

Description

technical field [0001] The invention belongs to the field of nanofiber membranes, in particular to a preparation method of a composite nanofiber membrane, a composite nanofiber membrane and its application in enzyme immobilization and immobilized enzymes. Background technique [0002] In recent years, the use of nanofibers as carriers to immobilize enzymes has become a major research hotspot at home and abroad. It mainly has the following advantages: (1) The amount of enzyme loaded is relatively large; (2) It can be separated from the product, which simplifies the purification process, increases the number of repeated use of the enzyme, and reduces the cost of the enzyme; (3) Can be used according to the carrier (4) It reduces the diffusion resistance of the substrate during enzyme catalysis, and the preparation of enzyme carriers with excellent performance is a research hotspot in enzyme immobilization technology. Electrospinning to prepare nanofibers has good performance, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D04H1/4382D01D5/00C12N11/087C12N11/10
CPCC12N11/08C12N11/12D01D5/003D04H1/4382D04H1/728
Inventor 刘新华宋远丁
Owner ANHUI POLYTECHNIC UNIV
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