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Method for determining inorganic negative ions in tobacco gene editing material

An inorganic anion, gene editing technology, applied in the field of analytical chemistry, can solve the problem that conventional methods are difficult to meet the demand, and achieve the effects of improving sample analysis efficiency, good compatibility, and shortening analysis time.

Active Publication Date: 2018-11-16
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To carry out chemical analysis and evaluation on these massive materials, conventional methods are difficult to meet the needs

Method used

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  • Method for determining inorganic negative ions in tobacco gene editing material
  • Method for determining inorganic negative ions in tobacco gene editing material
  • Method for determining inorganic negative ions in tobacco gene editing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Taking 4 kinds of finished tobacco leaves as inspection samples to determine the content of inorganic anions in them, including the following steps:

[0040] ①Sample pretreatment: select 4 finished tobacco leaves currently in use as the object of investigation, dry them in an oven at 40°C, then crush and pass through a 40-mesh sieve; then crush and pass through a 40-mesh sieve; accurately weigh 0.25g of the sample powder in the coat Add 25 mL of water as the extractant to the tube. Then, fix the inner sleeve of the extraction flask on the mouth of the outer sleeve with a sealing ring. After installation, put it in and place it in the tube and heat it for 20 minutes at 50℃; after the extraction is complete, Press down the inner sleeve of the extraction bottle forcefully, at this time the extraction solution enters the catheter through the filter sieve, and flows out of the catheter to achieve the effect of filtering the sample. Collect 1-1.5 mL of the extract from the downw...

Embodiment 2

[0047] The tobacco seedlings of 5 material samples from the gene editing breeding factory were selected as the object of investigation, and the samples were freeze-dried at -80°C. The sample processing and ion chromatography analysis were the same as in Example 1. The sample analysis results and evaluation are shown in Table 2.

[0048] Table 2 Analysis results of inorganic anions in tobacco products (mg / g)

[0049] sample

Embodiment 3

[0051] Taking 5 kinds of finished white tobacco leaves as investigation samples, determine the content of 6 kinds of inorganic anions among them. The determination procedure is the same as that in Example 1. The inorganic anion content in the samples is consistent with the range of high-quality white tobacco inorganic anion content.

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Abstract

The invention discloses a method for determining six inorganic negative ions in a tobacco gene editing material. An apparatus is used, which comprises a sample extraction bottle, an outer cannula (1),an inner cannula (2), a liquid-guiding pipe (4), and a sieve plate (3) which can be one or more and positioned in a lower opening of the inner cannula (2); The method comprises the following steps: 1) tobacco leaf extraction; 2) filtering and transferring; 3) analysis for making a chromatogram; 4) acquisition of a peak area of the standard chromatogram to obtain the inorganic negative ions of thetobacco gene editing material. The determination method of the present invention is characterized in that an on-line solid phase extraction purifying column is added before first usage of an ion chromatographic column, the processing is simple and convenient, the effect is good, the determination method has the advantages of accurate quantification, good repeatability, and low detecting limit, and can effectively realize the high flux analysis determination of the inorganic negative ions in tobacco gene editing material.

Description

Technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for measuring inorganic anions in tobacco gene editing materials. Background technique [0002] The inorganic anions in tobacco have important effects on the growth, development and quality of tobacco. Such as NO 3 - It is the main source of nitrogen during tobacco growth, Cl - It has an important impact on the moisture absorption of the leaves and the combustibility of tobacco leaves; SO 4 2- And PO 4 3- It is related to the assimilation of protein, sugar, and phospholipids during tobacco growth. NO 3 - And NO 2 - The content is also an important indicator reflecting nitrogen assimilation and the production of tobacco-specific nitrosamines. Therefore, the accurate determination of inorganic anions in tobacco is of great significance to the growth, development and quality evaluation of tobacco. [0003] The determination of inorganic anions usually adopts colorime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 李晶李天飞刘欣孔维松杨叶昆杨光宇黄海涛许永张涛张承明
Owner CHINA TOBACCO YUNNAN IND
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