Holly propagation method
A technology of holly and nutrient soil, applied in the direction of vegetative propagation, horticultural methods, botany equipment and methods, etc., can solve the problems of affecting germination rate and germination quality, long interval time, easy mildew of seeds, etc. The effect of shortening, increasing seedling rate, and high rooting rate
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Embodiment 1
[0018] The propagation method of holly specifically comprises the following steps:
[0019] Preparation of the extract: take 15 parts by weight of the residual leaves of holly, dry and pulverize, add 10 times of absolute ethanol containing α-terpineol, shake well, place in a 70°C water bath, heat for 8min, and then heat at a temperature of 40°C , ultrasonic extraction under the condition of 600W for 25min, filter to obtain the filtrate, repeat the extraction of the filter residue 2 times, mix and extract the obtained filtrate, and filter under reduced pressure to obtain the wintergreen extract, for subsequent use, the above-mentioned α-terpineol in The proportion by weight in absolute ethanol is 0.002%, and the ratio of L-isomer and D-isomer in α-terpineol is 100:45. Adding this ratio of α-terpineol to ultrasonic extraction can prevent on the one hand The fibrinogen in the plant cell wall becomes fibrin, so as to increase the elasticity of the cell wall and increase the effect...
Embodiment 2
[0026] The propagation method of holly specifically comprises the following steps:
[0027] Preparation of extract: Take 17 parts by weight of the residual leaves of holly, dry and pulverize, add 11 times of absolute ethanol containing α-terpineol, shake well, place in a water bath at 75°C, heat for 10 minutes, and then heat at 45°C , ultrasonic extraction under the condition of 650W for 35min, filter to obtain the filtrate, repeat the extraction of the filter residue 2 times, mix and extract the obtained filtrate, and filter under reduced pressure to obtain the wintergreen extract, for subsequent use, the above-mentioned α-terpineol in The proportion by weight in absolute ethanol is 0.003%, and the ratio of L-isomer and D-isomer in α-terpineol is 100:50;
[0028] Preparation of nutrient soil: select 13 parts of nursery soil, 10 parts of peat moss, 7 parts of zeatin, 10 parts of coir peat, 14 parts of vermiculite powder, and 7 parts of river sand by weight, and add water to ad...
Embodiment 3
[0034] The propagation method of holly specifically comprises the following steps:
[0035] Preparation of extract: Take 20 parts by weight of the residual leaves of holly, dry and pulverize, add 12 times of absolute ethanol containing α-terpineol, shake well, place in 80°C water bath, heat for 12min, and then heat at 50°C , under the condition of 750W ultrasonic extraction for 40min, filter to obtain the filtrate, repeat the extraction of the filter residue 3 times, mix and extract the obtained filtrate, and filter under reduced pressure to obtain the wintergreen extract, for subsequent use, the above-mentioned α-terpineol in The proportion by weight in absolute ethanol is 0.004%, and the ratio of L-isomer and D-isomer in α-terpineol is 100:55;
[0036] Preparation of nutrient soil: select 16 parts of nursery soil, 12 parts of peat moss, 9 parts of zeatin, 12 parts of coir peat, 16 parts of vermiculite powder, and 8 parts of river sand by weight, and add water to adjust the w...
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Abstract
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