Small dense low-density lipoprotein cholesterol kit

Abstract

The invention discloses a small dense low-density lipoprotein cholesterol kit. The kit consists of two independent reagents which are R1 and R2; the R1 contains a buffer solution, lipoprotein lipase,a nonionic surfactant, serum albumin, heparin sodium, magnesium ions, cholesteryl esterase, cholesterol oxidase, catalase or peroxidase, a Trinder's reactant A, a stabilizing agent and an antiseptic;and the R2 contains the buffer solution, the nonionic surfactant, a Trinder's reactant B, sodium azide and the antiseptic. The reagent R1 can be taken to mix with a specimen to eliminate cholesterol except for SD-LDLC; and reaction can be performed on the SD-LDLC after the reagent R2 is added, the Trinder's reactant B can be stained to determine absorbance, and content can be calculated. The kit is good in stability, high in accuracy, simple and fast in operation, and suitable for automatic biochemical analyzer detection.

Description

technical field [0001] The invention relates to a small and dense low-density lipoprotein cholesterol kit. Background technique [0002] "China Cardiovascular Disease Report 2016" pointed out that the number of cardiovascular disease patients in my country is about 290 million, accounting for more than 40% of the resident mortality rate, and the amount of cholesterol in the blood is too high, especially the bad cholesterol---low-density lipoprotein Cholesterol (LowDensity Lipoprotein Cholesterol, referred to as LDLC) is too high is one of the main causes of cardiovascular disease. [0003] Except for a small amount of free cholesterol in the blood, most of the cholesterol is combined with its corresponding protein to form lipoprotein cholesterol with different particle volumes and densities. In clinical testing, ultracentrifugation is commonly used to classify it as chylomicron (CM for short, particle size 80~ 500nm, density<0.95g / L), very low density lipoprotein (Very Lo...

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Problems solved by technology

[0006] SD-LDL is commonly determined by gradient gel electrophoresis (Gradient gel electrophoresis), proton NMR spectroscopy (Proton NMR spectroscopy), gradient ultracentrifugation (Density gradient ultracentrifugation), high pressure liquid chromatography (HPLC), etc., but the equipment is expensive and the operation is complicated. It is not suitable for routine inspection and determination. Although Hirano et al. invented that the turbidimetric reagent can be used for automatic biochemical analysis, the specimen must be processed first. After centrifuging lipoproteins other than SD-LDL, the supernatant is then used Nephelometric determination, troublesome operation, low specificity and accuracy

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