Bacillus safensis HMD9204 as well as fungicide and application thereof

A technology of bacillus saphobia and preparations, applied in the field of microorganisms, can solve problems such as economic loss, yield decline, aggravation of soil-borne diseases, etc., and achieve the effect of obvious control effect.

Active Publication Date: 2018-11-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the long-term planting of a certain crop year after year, serious secondary salinization of the soil and aggravation of soil-borne diseases have resulted in reduced crop quality and reduced yield, which has become an important obstacle restricting the sustainable development of facility agricu

Method used

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  • Bacillus safensis HMD9204 as well as fungicide and application thereof
  • Bacillus safensis HMD9204 as well as fungicide and application thereof
  • Bacillus safensis HMD9204 as well as fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and Identification of Sapphire Bacillus HMD9204

[0020] 1) Isolation of Bacillus suffolus HMD9204

[0021] The isolated soil in this example was collected from the saline soil (37°34'N 116°49'E) at the experimental base of the Chinese Academy of Agricultural Sciences in Ling County, Dezhou, Shandong. The five-point sampling method is used, that is, the midpoint of the diagonal line is first determined as the central sampling point, and then four points on the diagonal that are equal to the distance from the central sampling point are selected as sampling points. 1000m to the plot 2 The improved cultivated soil area was sampled, and the surface soil 15-20cm above the ground was taken for testing. The collected soil samples were divided into sample bags and recorded, and sent back to the laboratory in time.

[0022] Weigh 5 g of soil, add it to a conical flask (containing glass beads) filled with 45 ml of sterile saline, and vibrate in a constant t...

Embodiment 2

[0030] The inhibitory effect of embodiment 2 HMD9204 bacteria to Fusarium wilt of banana and Fusarium wilt of eggplant

[0031] Confrontation test: 3 groups were set up in the test: 1) Fusarium wilt of banana-Bacillus suffolus group; 2) Bacillus suffolus of eggplant wilt group; 3) control group. 3 repetitions per set. Punch a hole in the center of the nutrient broth medium with a sterile 5mm diameter puncher, and inoculate the pathogenic bacteria cake in the center. After cultivating for 24 hours, punch it with a sterile 5mm diameter punch on both sides at a distance of 3cm from the edge of the pathogenic bacteria cake in the center. Hole was punched, and Bacillus suffolus cake was inoculated, while the negative control group was inoculated with pathogenic bacteria, and the agar block was used to replace the Bacillus suffolus cake, placed in a 28°C incubator for 48 hours, and the inhibition zone was measured and calculated. Rate. The results are shown in Table 1.

[0032] T...

Embodiment 3

[0036]The bacillus fusarium HMD9204 powder provided by the invention is mixed with organic fertilizer or farmyard manure in proportion to the prevention and treatment effect of eggplant wilt and banana wilt is very obvious, in order to further give full play to its effect, determine its optimal mixing ratio, for It measures the effective viable count. The results are shown in Table 2.

[0037] Table 2 Detection of the number of effective viable bacteria mixed with HMD9204 powder and organic fertilizer

[0038]

[0039]

[0040] According to the test results of the effective number of viable bacteria mixed with HMD9204 bacterial agent and organic fertilizer, the effective number of viable bacteria in the powder / organic fertilizer ratio of 1.5:1000 can reach 100 million cfu / g.

[0041] The mixed use of embodiment 3HMD9204 powder and organic fertilizer on the influence of banana wilt etc.

[0042] This embodiment is set in the banana field in Hainan, which has been contin...

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Abstract

The invention discloses bacillus safensis HMD9204 as well as a fungicide and application thereof. A strain is preserved in the Guangdong microbial culture collection center on December 18th, 2017, with the collection number of GDMCC No.60302. The strain cooperates with organic fertilizer or farmyard manure to achieve a very obvious prevention and control effect on eggplant fusarium wilt and bananavascular wilt, reduces the incidence rate of the eggplant fusarium wilt to 2%-5%, and reduces the incidence rate of the banana vascular wilt to 6.7%-9.6%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Bacillus suffolus HMD9204 and its bacterial agent and application. Background technique [0002] Due to the long-term planting of a certain crop year after year, serious secondary salinization of the soil and aggravation of soil-borne diseases have resulted in reduced crop quality and reduced yield, which has become an important obstacle restricting the sustainable development of facility agriculture. Plant soil-borne diseases are an important class of plant diseases. There are many kinds of pathogens (fungi, bacteria, nematodes, viruses) that cause soil-borne diseases. They usually infect the roots of plants, causing diseases of plant roots and even the whole plant, causing major Economic losses. Among them, soil-borne pathogenic fungi have become one of the main restrictive factors for the stable and high yield of crops and vegetables. [0003] Banana wilt ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12R2001/07C12N1/205
Inventor 李春强彭明郝元福张辛耕杨景豪官小满
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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