Rabbit hemodisatal virus rod virus carrier, multi -killer Paramia disease two -couplet dying vaccine and preparation method

Abstract

The invention relates to a rabbit hemorrhagic disease virus baculovirus vector, a pasteurellosis multocida dual inactivated vaccine and a preparation method thereof, belonging to the technical field of immunization. Inoculate Sf9 insect cells with recombinant baculovirus of rabbit hemorrhagic disease virus VP60, culture at 27-28°C, harvest the cell culture and inactivate the cell culture when the pathological changes of the cells reach more than 85%, and use the inactivated product as the antigen of rabbit hemorrhagic disease virus; Pasteurella multocida C51-17 strain of capsular group A from rabbits was amplified and cultured, the bacterial liquid was inactivated, and the inactivated bacterial liquid was used as the Pasteurella multocida antigen; the two antigens and adjuvants were mixed according to Rabbit hemorrhagic disease virus baculovirus vector and pasteurellosis multocida dual inactivated vaccine are prepared by mixing in proportion. The present invention can provide a double inactivated vaccine of rabbit hemorrhagic disease virus baculovirus vector and pasteurellosis multocida with high safety, good immune effect and simple process, which is used for preventing and controlling rabbit viral hemorrhagic disease (rabbit plague) and pasteurellosis multocida of rabbits.

Description

Rabbit hemorrhagic virus baculovirus vector, pasteurellosis multocida combined inactivated vaccine Seedling and preparation method thereof technical field The present invention relates to a kind of rabbit hemorrhagic virus baculovirus vector, pasteurella multocida dual inactivated vaccine and The preparation method thereof belongs to the field of immunity technology. Background technique Rabbit viral hemorrhage (Rabbit hemorrhagic diease, RHD) is caused by rabbit hemorrhagic virus (Rabbit Hemorrhagic disease viurs, RHDV) is an acute, severe, high-contact infectious disease, commonly known as "rabbit The disease is often fulminant, with extremely high morbidity and mortality, with a mortality rate as high as 90%. Pasteurella multocida Pasteurellosis, also known as Hemorrhagic septicaemia (HS) in rabbits An acute, septicemic infection caused by Pasteurella multocida (Pm). The disease can be divided into nasal Inflammation type, pneumonia type, otitis media type ...

AI Technical Summary

Problems solved by technology

However, there are major defects in this vaccine, which are mainly manifested in that the individual animals used to prepare rabbit haemorrhagic disease virus are quite different, which is not conducive to the large-scale production of vaccine antigens, and the quality control is difficult to carry out. The most important thing is that the vaccine preparation process is easy to cause strong toxicity. Diffusion, poor biosafety

In addition, the preparation process of Pasteurella multocida is not stable enough, the antigen immunogenicity varies greatly between batches, and the challenge protection rate in the efficacy test is low

The above defects have seriously affected the quality of vaccines and restricted the production efficiency of enterprises

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