Multivariate volume column chip for visual detection of copper, lead and mercury ions and detection method thereof

A detection method and mercury ion technology, applied in the field of microfluidic analysis, can solve the problems of limited practical application, the detection limit of the method cannot meet the requirements of the national standard, and the number of metal ions is small, and the operation is simple, the test range is large, and the detection cost is achieved. low effect

Active Publication Date: 2022-02-11
NANJING UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these DNA-based visual detection can only give qualitative or semi-quantitative detection results, and the detection limit of the method cannot meet the requirements of the national standard, and instrumental methods are still needed
Moreover, the number of metal ions detected by these methods is too small, which limits the practical application.

Method used

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  • Multivariate volume column chip for visual detection of copper, lead and mercury ions and detection method thereof
  • Multivariate volume column chip for visual detection of copper, lead and mercury ions and detection method thereof
  • Multivariate volume column chip for visual detection of copper, lead and mercury ions and detection method thereof

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Embodiment

[0038] Such as Figure 1-3 As shown, a multi-component volume column chip for visually detecting copper, lead, and mercury ions includes a first glass chip 1 and a second glass chip 2, and the first glass chip 1 and the second glass chip 2 are rectangular and the size is Similarly, the inner side of the first glass chip 1 is bonded and slidably contacted with the inner side of the second glass chip 2, and the inner side of the first glass chip 1 is provided with an ink moving channel group, an ink indicating channel 3 and a sample tank group. The ink moving channel group is located on the upper part of the first glass chip 1, including the first ink moving channel 4, the second ink moving channel 5 and the third ink moving channel 6, the first ink moving channel 4, the second ink moving channel 5 and the third ink moving channel 6 are set parallel to each other from the left side to the right side of the first glass chip 1, and the sample tank group is located at the lower par...

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Abstract

The invention discloses a multi-volume column chip for visually detecting copper, lead and mercury ions, which comprises a first glass chip and a second glass chip, and an ink moving channel group, an ink indicating channel and a sample groove group are arranged inside the first glass chip , the inner side of the second glass chip is provided with a reagent tank group, and its detection method is also disclosed at the same time, including the preparation of a multivariate volume column chip, the preparation of nucleic acid modified magnetic silica nanoprobes, the preparation of DNA modified platinum nanoprobes, the preparation of composite There are six steps of probe, establishment of standard curve and sample detection; using multi-volume column chip to push the ink column to move through the oxygen generated by the decomposition of hydrogen peroxide, and present the detection result on the chip, without the need for expensive instruments and complicated data processing. It can realize visual and quantitative detection of the concentration of three metal ions of copper, lead and mercury in water, with accurate results, simple operation, low cost and wide application range.

Description

technical field [0001] The invention belongs to the technical field of microfluidic analysis, in particular to a multi-volume column chip for visually detecting copper, lead and mercury ions and a detection method thereof. Background technique [0002] At present, the detection of metal ions in water is mainly based on atomic absorption, atomic emission spectroscopy, and inductively coupled plasma mass spectrometry. These instruments can detect multiple metal ions at the same time, with high detection sensitivity and low detection limit. However, the equipment is expensive and requires high technical skills of the operator, which limits the application of on-site detection and resource-poor areas. [0003] In order to simplify the operation and reduce the cost, a visual detection method based on the metal-specific response of DNA was designed for the detection of metal ions. A series of specific DNA for metal ions can be produced through in vitro screening, which is used fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/6825
CPCC12Q1/6825C12Q2563/143C12Q2563/137C12Q2545/114C12Q2565/629
Inventor 宋玉君刘欣丽
Owner NANJING UNIV
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