A method for water quality monitoring using the ammonia entropy of aquatic organisms as an index

A technology of aquatic organisms and measurement methods, which is applied in the direction of testing water, material inspection products, etc., can solve the problems of inability to observe changes in physiological indicators of aquatic organisms and large data fluctuations, and achieve high practical value, real-time online detection, Simple calculation method

Active Publication Date: 2021-10-19
SHANDONG NORMAL UNIV
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  • Application Information

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Problems solved by technology

[0006] In water quality monitoring, if the water quality is monitored only through oxygen consumption rate or ammonia nitrogen excretion rate, the data fluctuates greatly, and it is impossible to observe the changes in the physiological indicators of aquatic organisms well.

Method used

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  • A method for water quality monitoring using the ammonia entropy of aquatic organisms as an index
  • A method for water quality monitoring using the ammonia entropy of aquatic organisms as an index
  • A method for water quality monitoring using the ammonia entropy of aquatic organisms as an index

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 aquatic organism oxygen consumption rate is measured

[0063] figure 2 It is a flow chart of a method for measuring the oxygen consumption rate of aquatic organisms applicable to this embodiment, such as figure 2 The methods for measuring the oxygen consumption rate of aquatic organisms shown include:

[0064] S1. Receive the oxygen concentration in the water measured by the aquatic organism metabolism measuring instrument, and use the average value of at least three odd-numbered slopes with weights to calculate the slope of the oxygen concentration over time;

[0065] S2. Select the range of data volume used when calculating the oxygen consumption rate from the received oxygen concentration data;

[0066] S3. According to the function of the slope of the oxygen concentration over time and the range of the data volume, calculate the average slope of the oxygen concentration and the time within a measurement time;

[0067] S4. Calculate the oxygen consu...

Embodiment 2

[0091] Determination of ammonia nitrogen content in water samples and calculation of aquatic organism ammonia nitrogen excretion rate in embodiment 2

[0092] image 3 Determination flow chart of ammonia nitrogen content in water samples, such as image 3 The methods for determining the content of ammonia nitrogen in the water samples shown include:

[0093] (i) Use two test tubes with a capacity of 10ml, one is filled with pure water and the other is filled with water sample, and the two kinds of water are added to the reaction tube;

[0094] (ii) Add 1 ml of N3 reagent to each tube;

[0095] (iii) continue to add 1 ml of N2 reagent to each tube of step (ii);

[0096] (iv) Shake each test tube in step (iii) after standing still for 10 minutes, and then take a colorimetric reading to calculate the ammonia nitrogen content.

[0097] The calculation method of ammonia nitrogen excretion rate of aquatic organisms is calculated by the following formula:

[0098]

[0099] Wh...

Embodiment 3

[0100] Embodiment 3 A method for monitoring water quality with aquatic organism ammonia entropy as an index

[0101] Described method comprises the following steps:

[0102] (1) The mensuration of oxygen consumption rate of aquatic organisms; Concrete assay method is as described in embodiment 1;

[0103](2) The mensuration of ammonia nitrogen content and the calculation of aquatic organisms ammonia nitrogen excretion rate in the water sample; Concrete measurement, calculation method are as described in embodiment 2;

[0104] (3) Calculation of the ammonia entropy of aquatic organisms; use the following formula to calculate:

[0105]

[0106] Among them, AQ is the ammonia entropy of aquatic organisms, VN is the ammonia nitrogen excretion rate of organisms, VO 2 is the oxygen consumption rate of aquatic organisms.

[0107] This experiment is a thallium nitrate exposure experiment, the experimental material is zebrafish, and the exposure concentration is the surface water ...

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Abstract

The invention relates to the technical field of water quality monitoring, in particular to a water quality monitoring method using the ammonia entropy of aquatic organisms as an index. The method comprises the following steps: (1) measuring oxygen consumption rate of aquatic organisms; (2) measuring ammonia nitrogen content in water samples and calculating ammonia nitrogen excretion rate of aquatic organisms; (3) calculating ammonia entropy of aquatic organisms. The invention utilizes the ammonia entropy of aquatic organisms to monitor the water quality, can directly and accurately reflect the change of the water quality, has a simple method for measuring and calculating the ammonia entropy, and is easy to realize real-time monitoring of the water quality.

Description

technical field [0001] The invention relates to the technical field of water quality monitoring, in particular to a method for monitoring water quality using the ammonia entropy of aquatic organisms as an index. Background technique [0002] With the rapid development of economy and society, and the acceleration of industrialization and urbanization, increasingly frequent human activities have aggravated the pollution of rivers and lakes. Aquatic organisms are an important part of the aquatic ecological environment. At present, in order to achieve the purpose of monitoring the water environment, changes in the structure of aquatic biological communities and changes in the physiological and biochemical conditions of aquatic organisms are usually used to directly reflect the impact and harm of water environment changes on organisms. Its purpose is to enable people to grasp the changes of the monitored water environment in a comprehensive and timely manner by quantitatively an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
CPCG01N33/18
Inventor 任宗明马静纯乔琳琳
Owner SHANDONG NORMAL UNIV
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