Novel mouse tail intravenous injection needle, manufacturing method thereof and experimental application

A tail vein injection and needle technology, applied in the field of new mouse tail vein injection needles and its manufacture, can solve the problems of puncturing the tail vein, easy slipping out, and reducing the range of twisting the body

Active Publication Date: 2018-12-28
TIANJIN GOALGEN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Advantages of this method: there is no need to purchase a mouse fixation device, saving costs; Disadvantages: During the process of puncturing and pushing the needle, the mouse tends to twist its body, resulting in unstable needle fixation, which can easily slip out or puncture the tail vein
Advantages of this method: The body parts other than the tail of the mouse are relatively fixed, which reduces the range of its twisting body to a certain extent, which is benefici

Method used

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  • Novel mouse tail intravenous injection needle, manufacturing method thereof and experimental application
  • Novel mouse tail intravenous injection needle, manufacturing method thereof and experimental application
  • Novel mouse tail intravenous injection needle, manufacturing method thereof and experimental application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Such as figure 1 A novel mouse tail vein injection needle shown includes a needle body 1, and the needle body 1 is fixedly connected with a thin sheet 2, and the thin sheet 2 is folded and pressed and fixed on the needle body, and a shape about the needle body is formed on the needle body. The left and right symmetrical square side wings 3 of the needle body, one end of the side wing 3 is located at the midpoint of the needle body 1 , and the other end is located at the bottom of the needle body 1 .

[0027] In this embodiment, the length of the side wings 3 is 50% of the length of the needle body, and according to actual needs, it is between 45% and 55%, preferably 50%. Within this ratio range, it can effectively ensure the smooth insertion of the needle .

[0028] In this embodiment, the width of the left and right ends of the wing 3 is 2mm, of course, it can fluctuate between 1.9-2.1mm according to actual needs, preferably 2mm.

[0029] In this embodiment, the shee...

Embodiment 2

[0031] Using the novel mouse tail vein injection needle mentioned in Example 1 to carry out breast cancer cell metastasis experiment on mutant mice, the specific operation steps are as follows:

[0032] 1) Divide 32 female SPF Scid mice into two groups of CON and MU, 16 in each group, weighing 14-16g, and cut their ears respectively for marking.

[0033] 2) The human breast cancer cell line MDA-MB-231 and its mutant cell line MDA-MB-231MU were respectively configured as 5×10 6 / ml of cell suspension.

[0034] 3) Inject 0.2ml of MDA-MB-231 cell suspension into the tail vein of each CON group mouse, among which, 8 mice are injected with a disposable 1ml syringe with a traditional medical 25G injection needle, and the other 8 mice are injected with a disposable 1ml syringe Inject with a new type of needle, record the injection time (unit: s) of each mouse, and compare the twisting range of the mouse body and the success rate of injection when the two needles are injected.

[00...

Embodiment 3

[0046] Using the novel mouse tail vein injection needle mentioned in Example 1 to carry out breast cancer cell metastasis experiment on mutant mice, the specific operation steps are as follows:

[0047]1) Divide 32 female nude mice of SPF grade into two groups of CON and MU, with 16 mice in each group, weighing 14-16g, and cut their ears respectively for marking.

[0048] 2) The human breast cancer cell line MCF-7 and its mutant cell line MCF-7MU were respectively configured as 8×10 6 / ml of cell suspension.

[0049] 3) Each mouse in the CON group was injected with 0.2ml of MCF-7 cell suspension into the tail vein, 8 of them were injected with a disposable 1ml syringe with a traditional medical 25G injection needle, and the other 8 were injected with a disposable 1ml syringe with a new For needle injection, the injection time (unit: s) of each mouse was recorded respectively, and the twisting range of the mouse body and the success rate of bolus injection were compared betwee...

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Abstract

The invention relates to the technical field of applications of experimental mice to in-vivo experiments, and particularly discloses a novel mouse tail intravenous injection needle, a manufacturing method thereof and an experimental application. The novel injection needle is used for mouse tail intravenous injection. On the basis of an existing medical 25G syringe needle, bilaterally symmetric side wings are added downwards from the midpoint of the needle to manufacture the novel needle. The novel needle is used for mouse tail intravenous injection, so that mouse tail intravenous injection success rate can be greatly increased. Compared with the traditional medical 25G syringe needle, the novel needle has the advantages that the novel needle is used for performing tail intravenous injection experiments on the mice, needle angles and needle lengths can be effectively controlled, and punching failure or piercing of tail veins caused by incorrect needle angles is maximally avoided.

Description

technical field [0001] The invention relates to the technical field of using experimental mice for in vivo experiments, in particular to a novel mouse tail vein injection needle and its manufacturing method and experimental application. Background technique [0002] Experimental mice are small in size, not aggressive, and easy to feed and operate. They are one of the most commonly used experimental animals for in vivo experiments. They are often used in experiments such as pharmacology, toxicology, tumors, immunity, and biochemistry. The experimental methods of mice mainly include intragastric administration, intraperitoneal injection, subcutaneous injection, tail vein injection, etc. Among them, tail vein injection is an important and commonly used experimental method. However, because the tail vein of the mouse is thin, it is difficult to puncture. During the injection process, the mouse twisted its body due to pain, causing the needle to slip out or puncture the tail vein...

Claims

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Application Information

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IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 冀为梁兰王菁侯蔺桐
Owner TIANJIN GOALGEN BIOTECH
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