Microorganism solid phase-free drilling fluid and preparation method thereof
A technology without solid phase and drilling fluid, which is applied in the direction of chemical instruments and methods, drilling compositions, etc., and can solve problems such as ineffectiveness, lack, and lack of deep involvement
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[0027] (1) Preparation of Bacillus pasteurian bacteria liquid
[0028] ① Culture medium production
[0029] First, measure 1000ml of pure water with a graduated cylinder and add it to the beaker. Then take by weighing soy peptone 5g, casein peptone 15g, sodium chloride 5g successively with electronic balance. After weighing, slowly pour it into the beaker, and at the same time stir quickly with a glass rod to ensure that the nutrients are completely dissolved in pure water. The pH was adjusted to 7.3 after thorough stirring.
[0030] ② Medium sterilization
[0031] Use a pipette gun to transfer 100ml of medium into the Erlenmeyer flask, seal the mouth of the Erlenmeyer flask with kraft paper, put it in a high-pressure steam cooker for sterilization, and take it out after 15 minutes of sterilization at 0.1MPa and 110°C. Put it on the ultra-clean workbench to cool naturally.
[0032] ③ Strain inoculation
[0033] First activate the strains of Bacillus pasteurianum, burn th...
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