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Fully-sealed microfluidics chip and emulsion microdroplet preparation system

A technology of microfluidic chip and preparation system, which is applied in heating or cooling equipment, chemical instruments and methods, laboratory utensils, etc., and can solve problems affecting the accuracy of measurement results, emulsion droplet movement loss, emulsion droplet breakage, etc. , to achieve the effect of facilitating rapid promotion, avoiding aerosol pollution, and reducing professional requirements

Pending Publication Date: 2019-01-01
长春技特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When reading the results, it is necessary to take out the emulsion droplets from the 96-well plate. In the above process, the emulsion droplets will be damaged, the movement loss of the emulsion droplets, and even aerosol pollution, resulting in false positives and affecting Accuracy of Measuring Effects

Method used

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  • Fully-sealed microfluidics chip and emulsion microdroplet preparation system
  • Fully-sealed microfluidics chip and emulsion microdroplet preparation system
  • Fully-sealed microfluidics chip and emulsion microdroplet preparation system

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Embodiment 1

[0034] The present invention will be further described below in conjunction with accompanying drawing:

[0035] Such as Figure 4 , 5 , 6, 1 is the capacitive touch screen, 2 is the LED indicator light, 3 is the translucent protective cover, 4 is the chip pressure plate, 5 is the overall shell, 6 is the compression pump, 7 is the gas cylinder, 8 is the power supply Module, 9 is the valve array, 10 is the control circuit, 11 is the interface A closely connected with the oil phase inlet, 12 is the interface B closely connected with the sample inlet, 13 is the interface C closely connected with the vent hole, 14 Is gland rotating shaft, 15 is pipeline connection.

[0036] Such as figure 1 , 2 , 3, 16 is the emulsion droplet generation structure area, 17 is the PCR structure area, 18 is the oil phase introduction pipeline, 19 is the oil phase introduction port, 20 is the sample introduction port, 21 is the cavity, 22 is the sealing film , 23 is a droplet collection cavity, 24...

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Abstract

The invention relates to a fully-sealed microfluidics chip and an emulsion microdroplet preparation system, and belongs to the technical field of biochemical equipment. The fully-sealed microfluidicschip is characterized by comprising an emulsion microdroplet generation structure and several to dozens of PCR (polymerase chain reaction) structures, wherein the emulsion microdroplet generation structure is used for generating a water-in-oil emulsion microdroplet by an oil phase and a sample water phase; each PCR structure is of a sealed structure. The preparation system comprises a control instrument, an emulsion microdroplet device and the microfluidics chip. The microfluidics chip has the beneficial effects that 1, the emulsion microdroplet generation structure and the PCR structures areintegrated into one unit, so that the professional requirement on an operator is decreased by the integral design, and the technique can be quickly popularized; 2, the possibility of introducing pollution by PCR is decreased, and the accuracy in digital PCR detection is improved; 3, the pollution to an aerosol is avoided; 4, the damage to the emulsion microdroplet in the PCR process is avoided; 5,the control instrument is provided with a plurality of working positions for setting the microfluidics chips, such as two working positions; 6, the operation is simplified, the time is shortened, andthe efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of biochemical test detection equipment. Background technique [0002] The digital PCR technology uses the water-in-oil emulsion micro-droplet as the micro-reactor of the PCR reaction for PCR amplification. The biggest feature of digital PCR is that it can form a large number of independent reaction spaces for PCR amplification. Microemulsion droplets formed from oil-water two-phase have been used in high-throughput analysis systems, and their application range has a tendency to expand. For example, emulsion droplets are formed for polymerase chain reaction (PCR) binding, and a single copy of a nucleic acid target molecule is amplified in the droplets, thereby realizing the detection of the existence of a single molecule of the target and the specific gene sequence. 10X Genomics, an American company, uses emulsion droplet PCR method combined with Gemcode technology to generate long-chain DNA, thus realizin...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L2300/0809B01L7/52
Inventor 褚春旭司远吕洋
Owner 长春技特生物技术有限公司
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