Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting content and structure of resistant starch of potato on basis of field-flow fractionation technology

A technology of potato starch and resistant starch, which is applied in the direction of scattering characteristic measurement, phase influence characteristic measurement, test sample preparation, etc. It can solve the problem of high cost, long time consumption, and inability to provide resistant starch rotation radius and molecular weight distribution information at the same time and other issues to achieve the effect of short time-consuming and low cost

Active Publication Date: 2019-01-04
HEBEI UNIVERSITY
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting the content and structure of potato resistant starch based on field flow separation technology, so as to solve the problem of high cost and time-consuming detection of potato resistant starch content by AOAC kit method, and the inability to provide resistant starch at the same time Problems with Radius of Rotation and Molecular Weight Distribution Information

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting content and structure of resistant starch of potato on basis of field-flow fractionation technology
  • Method for detecting content and structure of resistant starch of potato on basis of field-flow fractionation technology
  • Method for detecting content and structure of resistant starch of potato on basis of field-flow fractionation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The method for detecting the content and structure of potato resistant starch based on field flow separation technology provided by the present invention comprises the following steps:

[0035] 1. Hydrolyzed potato non-resistant starch

[0036] (1) Accurately weigh 100 mg of potato starch into a 20 mL sample bottle, add 6 mL of 0.1 M sodium acetate buffer (pH 5.2, containing 1 mM CaCl 2 ). After tightening the screw cap of the sample bottle, place it in a 37°C water bath to preheat for 5 min.

[0037] (2) Add 4.0 mL of enzyme mixture (containing α-trypsin (142 U / mL) and AMG (15 U / mL)) to the sample bottle.

[0038] (3) Stir continuously at 160 rpm and incubate at 37°C for 2 h.

[0039] (4) Transfer the sample vial from a 37°C water bath to a 90°C water bath, and let it stand for 2 minutes to inactivate the digestive enzymes.

[0040] (5) Take the sample bottle out of the 90°C water bath and let it cool down to room temperature naturally.

[0041] 2. Dissolve potato...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting content and a structure of resistant starch of a potato on the basis of field-flow fractionation technology. The method includes: a, hydrolyzing non-resistant starch of the potato; b, dissolving the resistant starch of the potato; c, measuring the content and the structure of the resistant starch of the potato by field-flow fractionation; d, calculating the content of the resistant starch of the potato. A multi-angle laser light scattering detector and a differential refraction detector are combined with asymmetric field-flow fractionation to detect the content of the resistant starch of the potato, the whole detection process is short and only lasts for 4 hours, and a turning radius and molecular weight distribution of the resistant starch canbe provided at the same time; the method is low in cost since an aqueous solution of 50 mM NaNO3+3 mM NaN3 is adopted as a flowing phase; the method has remarkable advantages as compared with a present universal AOAC (Association of Official Analytical Chemists) resistant starch detection method.

Description

technical field [0001] The invention relates to a method for detecting potato resistant starch, in particular to a method for detecting the content and structure of potato resistant starch based on field flow separation technology. Background technique [0002] With the improvement of people's living standards, more and more attention has been paid to eating high-nutrition, low-calorie functional foods. Starch is an important raw material in the food industry. According to its digestion rate and degree in the body, it is divided into three categories: fast-digesting starch, slow-digesting starch and resistant starch. Resistant starch is a low-calorie, dietary fiber-like functional food ingredient. It has similar functions to dietary fiber and is superior to dietary fiber in terms of taste and certain properties. It helps prevent diabetes, control weight, and improve the body. Immunity, prevention of colon cancer and other functions. [0003] Potatoes are the fourth largest...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/51G01N21/41G01N1/38
CPCG01N1/38G01N21/41G01N21/51
Inventor 窦海洋张文惠李月秋申世刚
Owner HEBEI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com