An adaptive scanning wide-field high-throughput tomographic imaging method and device

A micro-imaging, wide-field-of-view technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as increasing excitation light power, and achieve the effects of reducing light damage, reducing power level, and high excitation efficiency
CN109187459BActive Publication Date: 2020-06-16TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2020-06-16

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Abstract

The invention discloses a method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput, and belongs to the technical field of microimaging. According tothe method, the target imaging area of a sample is divided into a plurality of sub-areas by utilizing ultrashort pulse laser through a method of combining spatial and temporal focusing and scanning, each sub-area is quickly and adaptively scanned by adopting a time division multiplexing method, synchronous data collection is carried out, reconstruction and data processing are performed on collected microimages in each sub-area to obtain a three-dimensional spatial message of the target scanning area in a scanning cycle, and the four-dimensional information of a sample (x, y, z and t) is acquired through three-dimensional spatial scanning and delayed scanning. The device comprises an ultrashort pulse laser light source and beam conversion system, a sub-area scanning system combined with thespatial and temporal focusing technology, an optical microscopic and filter system, a synchronous microimaging system and an image reconstruction and data processing system. The device has the advantages of wide view field, high throughput, low exciting power, high signal-to-noise ratio and the like.
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Description

technical field

[0001] The invention relates to an adaptive scanning wide-field high-throughput tomographic microscopic imaging method and device, and belongs to the technical field of microscopic imaging. Background technique

[0002] Systems biology research, especially the study of biological dynamic processes, usually requires large-scale, high-spatial-resolution observations. For example, in brain science, in order to study the transmission and transformation of nerve impulses in neural networks (such as neural activity across brain regions, etc.); in immunology and oncology, in order to study the transfer or migration of immune cells or tumor cells, etc. , all require large-scale, high-resolution, high-speed, high-throughput in-situ observations in vivo.

[0003] However, there is an inherent contradiction between large field of view and high resolution in objective lenses of commercial microscope systems. To this end, several new designs of Mesoscopes have been prop...

Claims

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