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SFTS recombinant NP protein nano-particle based immunological detection method

An immunodetection method and nanoparticle technology, applied in the field of immunodetection, can solve the problems of low detection sensitivity, defects and high false negative rate, and achieve the effects of high detection sensitivity, high accuracy and low false negative rate.

Inactive Publication Date: 2019-01-15
武汉优恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Although the colloidal gold immunochromatography reagents on the market are simple and low-cost, they are suitable for on-site rapid detection, but the characteristics of the gold particle tracer in the method determine the defects of the detection system, the detection sensitivity is low, and the false negative rate is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An immunological detection method based on SFTS recombinant NP protein nanoparticles, the specific detection method steps are as follows:

[0026] Step 1: Use up-converting nanoparticles to label the recombinant NP protein coated with SFTS, and then add 0.02-0.06 mg / ml lectin diluent to prepare the detection and analysis solution;

[0027] Step 2: Mix the serum to be tested and the detection and analysis solution at a ratio of 1:1, and add 100 μl of the mixed solution to the sampling hole of the test card;

[0028] Step 3: Use a fluorescence scanning detector to detect the mixed liquid in step 2, and calculate the size of the error;

[0029] Step 4: On the basis of the normality in Step 3, use fluorescence immunochromatography to detect 100 cases of healthy human serum;

[0030] Step 5: Transmit the detection result of step 4 to the microcontroller controller to read the T / C value, and calculate the mean (A), standard deviation (SD) and Cutoff value;

[0031] Step 6: ...

Embodiment 2

[0040] An immunological detection method based on SFTS recombinant NP protein nanoparticles, the specific detection method steps are as follows:

[0041] Step 1: Use fluorescein to label the recombinant NP protein coated with SFTS, and then add 0.02-0.06mg / ml phytohemagglutinin dilution solution to prepare the detection analysis solution;

[0042] Step 2: Mix the serum to be tested and the detection and analysis solution at a ratio of 1:1, and add 100 μl of the mixed solution to the sampling hole of the test card;

[0043] Step 3: Use a fluorescence scanning detector to detect the mixed liquid in step 2, and calculate the size of the error;

[0044] Step 4: On the basis of the normality in Step 3, use fluorescence immunochromatography to detect 100 cases of healthy human serum;

[0045] Step 5: Transmit the detection result of step 4 to the microcontroller controller to read the T / C value, and calculate the mean (A), standard deviation (SD) and Cutoff values;

[0046] Step 6...

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Abstract

The invention discloses an SFTS recombinant NP protein nano-particle based immunological detection method. The method comprises the following specific detection steps: (1) respectively labeling SFTS recombinant NP protein for coating by using UCNPs, and then adding phytohemagglutinin diluent of 0.02-0.06 mg / ml to prepare detection analysis liquid; (2), uniformly mixing to-be-detected serum with the detection analysis liquid at a ratio of 1 : 1, and adding a mixed liquid of 100 microlitres to sample application holes of a detection card; (3), detecting the mixed liquid in step (2) by utilizinga fluorescence scanning detector to calculate the error; (4), based on the normal step (3), detecting 100 parts of healthy human serum by utilizing a fluorescence immunochromatographic assay method; and (5), transmitting the detection result in the step (5) to a single-chip computer controller to read T / C value, and calculating an average value (A), a standard deviation (SD) and a Cutoff value. The SFTS recombinant NP protein nano-particle based immunological detection method has the advantages of high detection speed, accurate data processing, high practicability, convenience in production and wide popularization and application.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to an immunological detection method based on SFTS recombinant NP protein nanoparticles. Background technique [0002] Although the colloidal gold immunochromatography reagents on the market are simple and low-cost, they are suitable for on-site rapid detection, but the characteristics of the gold particle tracer in the method determine the defects of the detection system, the detection sensitivity is low, and the false negative rate is high . SFTSV is a natural foci disease, which usually occurs in areas with relatively poor medical and health conditions, such as mountainous areas and hills. The level of medical and health care is low, and the concept of seeking medical treatment is poor, which greatly affects the early diagnosis and treatment of the disease. Contents of the invention [0003] The object of the present invention is to provide a kind of immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 冷毅斌胡勤芹夏红星冯亮
Owner 武汉优恩生物科技有限公司
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