A kind of SNP molecular marker of wax gourd seed type gene and its application

A technology of molecular markers and wax gourd seeds, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., to achieve the effects of strong specificity, easy operation, and short identification cycle
CN109234433BActive Publication Date: 2019-06-21INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
Publication Date
2019-06-21

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Abstract

The invention discloses an SNP molecular marker of a wax gourd seed type gene. The nucleotide sequence of the molecular marker is shown in SEQ ID NO. 1. The nucleotide sequence shown in SEQ ID NO. 1 is a SNP site from the 5 'end, and the nucleotide of the nucleotide sequence is T or C. Primer pairs for amplifying the SNP molecular markers described above are also disclosed, the pair of primers comprising an upstream primer and a downstream primer, The nucleotide sequence of the upstream primer is shown in SEQ ID NO. 2, and the nucleotide sequence of the downstream primer is shown in SEQ ID NO.3. The primer has good repeatability and high specificity for producing unilateral seed material specific marker and bilateral seed material specific marker; and the nucleotide sequence of the downstream primer is shown in SEQ ID NO. Also disclosed is a method for identifying wax gourd seed type, which is accurate, rapid, low in cost, short in identification period and simple in operation. Further disclosed are the application of the SNP molecular marker and the primer pair in identifying the seed type of winter gourd.
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Description

technical field

[0001] The invention belongs to the technical field of molecular markers, and in particular relates to a SNP molecular marker of a wax gourd seed type gene and an application thereof. Background technique

[0002] According to the shape and structure of the wax gourd seed coat, the mature seeds can be divided into non-prismatic seeds (commonly known as single-sided seeds) and ribbed flat seeds (commonly known as double-sided seeds). Unilateral seeds usually have different degrees of dormancy, and their germination rate is generally below 60%, and they germinate slowly and irregularly, while bilateral seeds germinate quickly and neatly, and their germination rate exceeds 90%. According to the national seed standard, the germination rate of winter melon commercial seeds is only 60%, while the germination rate standard of other cucurbit crops is above 90%. Therefore, seed type is an important trait of winter melon, and double-seed varieties are favored by seed ...

Claims

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