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Hydrogel with adjustable structure size, preparation method and application thereof

A technology of structural size and hydrogel, applied in medical science, prosthesis, etc., can solve the problems of obtaining hydrogel, uncontrollable structural size, and inability to fully adapt to the growth of seed cells, so as to enhance drug efficacy and facilitate repair Effect

Inactive Publication Date: 2019-01-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The silk fibroin hydrogel provided by this invention has the characteristics of high strength, elasticity and transparency, but its structural size is uncontrollable, and it is impossible to obtain hydrogels of corresponding sizes according to specific experimental requirements
[0006] It is precisely because of the three-dimensional structure of hydrogels that hydrogels have attracted widespread attention. However, the structural size of existing hydrogels is generally uncontrollable, and as tissue repair materials, they cannot fully adapt to the growth of seed cells.

Method used

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  • Hydrogel with adjustable structure size, preparation method and application thereof
  • Hydrogel with adjustable structure size, preparation method and application thereof
  • Hydrogel with adjustable structure size, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Preparation of a hydrogel with adjustable structural size

[0037] A method for preparing a hydrogel with adjustable structural size, comprising the following steps:

[0038] S1 Dissolve 0.9g of gelatin in 9mL of deionized water, heat to 70°C to dissolve the gelatin to obtain a gelatin solution with a mass volume percentage (g / mL) of 10%, and pour the gelatin solution into a beaker filled with ethyl acetate , set the rotation speed at 300r / min, and stir for 5min to obtain a gelatin / ethyl acetate uniform emulsion, wherein the volume ratio of gelatin solution to ethyl acetate is 3:2;

[0039] S2 Pour the gelatin / ethyl acetate homogeneous emulsion obtained in step S1 into a beaker filled with edible olive oil, set the speed at 200r / min, stir for 5min, then soak the entire beaker in ice water, set the speed at 500r / min , after stirring for 20 minutes, an oily emulsion was obtained, wherein the volume ratio of edible olive oil to gelatin / ethyl acetate homogeneo...

Embodiment 2

[0045] Embodiment 2 Preparation of a hydrogel with adjustable structural size

[0046] A method for preparing a hydrogel with adjustable structural size, comprising the following steps:

[0047] S1 Dissolve 3g of gelatin in 30mL of deionized water, heat to 70°C to dissolve the gelatin to obtain a gelatin solution with a mass volume percentage (g / mL) of 10%, pour the gelatin solution into a beaker equipped with ethyl acetate, Set the rotation speed at 400r / min, and stir for 1min to obtain a gelatin / ethyl acetate homogeneous emulsion, wherein the volume ratio of gelatin solution to ethyl acetate is 15:2;

[0048] S2 Pour the gelatin / ethyl acetate homogeneous emulsion obtained in step S1 into a beaker filled with edible olive oil, set the speed at 1200r / min, stir for 1min, then soak the entire beaker in ice water, set the speed at 600r / min , after stirring for 5 minutes, an oily emulsion was obtained, wherein the volume ratio of edible olive oil to gelatin / ethyl acetate homogeneou...

Embodiment 3

[0054] Example 3 Preparation of a hydrogel with adjustable structural size

[0055] A method for preparing a hydrogel with adjustable structural size, comprising the following steps:

[0056] S1 Dissolve 1.2g of gelatin in 12mL of deionized water, heat to 70°C to dissolve the gelatin to obtain a gelatin solution with a mass volume percentage (g / mL) of 10%, and pour the gelatin solution into a beaker filled with ethyl acetate , set the rotation speed at 500r / min, stir for 3min to obtain a gelatin / ethyl acetate homogeneous emulsion, wherein the volume ratio of gelatin solution to ethyl acetate is 12:2;

[0057] S2 Pour the gelatin / ethyl acetate homogeneous emulsion obtained in step S1 into a beaker filled with edible olive oil, set the speed at 900r / min, stir for 3min, then soak the entire beaker in ice water, set the speed at 700r / min , after stirring for 15 minutes, an oily emulsion was obtained, wherein the volume ratio of edible olive oil to gelatin / ethyl acetate homogeneou...

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Abstract

The invention discloses a hydrogel with adjustable structure size, a preparation method and an application thereof, belonging to the field of biomedical materials. The preparation method of the hydrogel comprises the following steps: firstly, gelatin microspheres with different sizes were prepared, then the gelatin microspheres were immersed in the solution containing growth factors or drugs to obtain drug-loaded gelatin microspheres, and then the drug-loaded gelatin microspheres were mixed with the desired hydrogel, and seeded with the desired seed cells to obtain the hydrogel with adjustablestructure size. The size of the hydrogel prepared by the method can be adjusted according to the practical application, and the specific function can be obtained by combining the required growth factors or drugs, and the gel matrix material is suitable for the growth of the specific seed cells, and finally the hydrogel with the specific tissue repair function is prepared, and the hydrogel has a broad application prospect in the tissue engineering repair.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a hydrogel with adjustable structural size and its preparation method and application. Background technique [0002] Hydrogel is a gel with water as the dispersion medium. It is a water-soluble polymer with a network crosslinked structure that introduces a part of hydrophobic groups and hydrophilic residues. The hydrophilic residues combine with water molecules to connect water molecules. In the interior of the network, the cross-linked polymer with hydrophobic residues that swell with water is a polymer network system that is soft in nature, can maintain a certain shape, and can absorb a large amount of water. [0003] Many tissues in organisms have a hydrogel structure. Biological tissues are composed of cells and extracellular matrix, and the extracellular matrix components such as proteins and polysaccharides form a hydrogel-like structure. Therefore, from the...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54A61L27/56
CPCA61L27/20A61L27/222A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/414A61L2300/416C08L5/04C08L5/08
Inventor 曾蕾陈晓峰曹晓东王刚
Owner SOUTH CHINA UNIV OF TECH
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