Tetracyclic triterpenoid compounds and preparation method thereof

A technology for tetracyclic triterpenes and compounds, which is applied in the field of tetracyclic triterpenoids and their preparation, can solve the problems of complex process, long cycle, and low purity of balsam pear, and achieves simple sample preparation, simple operation, and fast operation. efficient effect

Inactive Publication Date: 2019-01-25
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Abstract
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Problems solved by technology

[0006] At present, most of the research on the separation and purification of Momordica charantin at home and abroad is still in the laboratory research stage. The separation and purification method mainly adopts the method of combining liquid-liquid extraction and classical column chromatograp...

Method used

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  • Tetracyclic triterpenoid compounds and preparation method thereof
  • Tetracyclic triterpenoid compounds and preparation method thereof
  • Tetracyclic triterpenoid compounds and preparation method thereof

Examples

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Embodiment 1

[0055] (1) Preparation of tetracyclic triterpenoids

[0056] Bitter gourd is planted in the experimental farm of the School of Resources and Environment of South China Agricultural University. After the bitter gourd is harvested, the leaves of bitter gourd are collected.

[0057] The bitter gourd leaves are dried and crushed to obtain the bitter gourd leaves coarse powder, which is placed in a multifunctional extractor, and a 90% ethanol solution of 15 times the sample amount is added, soaked at a constant temperature of 50°C for 24 hours, the filtrate is exuded, and a total of 3 extractions are performed. Combine the filtrates.

[0058] The filtrate was concentrated under reduced pressure on a rotary evaporator to obtain a crude ethanol extract, which was then extracted by the conventional "liquid-liquid extraction method", extracted with petroleum ether, ethyl acetate and n-butanol, combined the extracts, and obtained by distillation under reduced pressure. Petroleum ether, ethyl ...

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Abstract

The invention discloses tetracyclic triterpenoid compounds and a preparation method thereof. Bitter gourd leaves are adopted as a raw material, then subjected to 90% ethanol reflux extraction to be concentrated into an extract and then subjected to extraction with ethyl acetate, silica gel column chromatography separation, high performance liquid chromatography preparation and magnetic resonance imaging structural identification to successfully extract and separate the 4 kinds of tetracyclic triterpenoid compounds with the purity of more than 95%. According to the preparation method, the high-purity tetracyclic triterpenoid compounds can be separated and purified. The method is simple, feasible, good in reproducibility, fast and efficient, high in product purity and suitable for separationand preparation of various types of natural compounds from the bitter gourd leaves, thereby laying a technical foundation for screening active compounds from the bitter gourd leaves.

Description

Technical field [0001] The invention relates to the field of phytochemistry, in particular to a tetracyclic triterpenoid compound and a preparation method thereof. Background technique [0002] Momordica charantia (Momordica charantia L.), also known as Jinlichee and Bitter Melon, is an annual climbing herb of the Cucurbitaceae Momordica genus (Momordica). As a medicinal and food-homogenous melon vegetable, it can enhance immunity, resist diabetes, Anti-tumor, weight loss and antibacterial effects (Grover and Yadav, 2004; Beloin et al., 2005; Maurya et al., 2009). [0003] Compared with other cucurbitaceous crops, bitter gourd is rarely harmed by herbivorous insects during growth and development, and shows strong insect resistance. A large number of research results show that momordicins in the stems and leaves of bitter melon are the main anti-insect active substances. Momordicae is a kind of cucurbitane-type tetracyclic triterpeoids (tetracyclic triterpeoids). According to the ...

Claims

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Application Information

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IPC IPC(8): C07J17/00C07J9/00
CPCC07J9/00C07J17/005
Inventor 刘欢凌冰张茂新安玉兴赵欢欢付建涛孙东磊卢颖林陈立君龚恒亮
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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