Tetracyclic triterpenoid compounds and preparation method thereof

Abstract

The invention discloses tetracyclic triterpenoid compounds and a preparation method thereof. Bitter gourd leaves are adopted as a raw material, then subjected to 90% ethanol reflux extraction to be concentrated into an extract and then subjected to extraction with ethyl acetate, silica gel column chromatography separation, high performance liquid chromatography preparation and magnetic resonance imaging structural identification to successfully extract and separate the 4 kinds of tetracyclic triterpenoid compounds with the purity of more than 95%. According to the preparation method, the high-purity tetracyclic triterpenoid compounds can be separated and purified. The method is simple, feasible, good in reproducibility, fast and efficient, high in product purity and suitable for separationand preparation of various types of natural compounds from the bitter gourd leaves, thereby laying a technical foundation for screening active compounds from the bitter gourd leaves.

Description

Technical field [0001] The invention relates to the field of phytochemistry, in particular to a tetracyclic triterpenoid compound and a preparation method thereof. Background technique [0002] Momordica charantia (Momordica charantia L.), also known as Jinlichee and Bitter Melon, is an annual climbing herb of the Cucurbitaceae Momordica genus (Momordica). As a medicinal and food-homogenous melon vegetable, it can enhance immunity, resist diabetes, Anti-tumor, weight loss and antibacterial effects (Grover and Yadav, 2004; Beloin et al., 2005; Maurya et al., 2009). [0003] Compared with other cucurbitaceous crops, bitter gourd is rarely harmed by herbivorous insects during growth and development, and shows strong insect resistance. A large number of research results show that momordicins in the stems and leaves of bitter melon are the main anti-insect active substances. Momordicae is a kind of cucurbitane-type tetracyclic triterpeoids (tetracyclic triterpeoids). According to the ...

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Problems solved by technology

[0006] At present, most of the research on the separation and purification of Momordica charantin at home and abroad is still in the laboratory research stage. The separation and purification method mainly adopts the method of combining liquid-liquid extraction and classical column chromatography, but the purity of Momordica charantin obtained in this way is not very high , often contain other impurities with similar structures, and the process is complex and the cycle is long (Shi Xueping, 2006; Ren Liyun et al., 2008; Cheng Lanying et al., 2008; Wang Hu et al., 2011; Li Wen et al., 2012)

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