Preparation method of high purity and low density lipoprotein
A low-density lipoprotein, high-purity technology, applied in the field of material extraction, can solve the problems of less conventional extraction, affecting dialysis efficiency, and difficulty in meeting the requirements of blood collection, and achieve the effect of improving the recovery rate.
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[0028] The present invention will be further described below based on the drawings and embodiments.
[0029] 1. Plasma separation:
[0030] Ethylenediaminetetraacetic acid (EDTA) is added to the plasma, the final concentration is 1mmol / L, 3000rpm×20min, the precipitation is blood cells, and the supernatant plasma is ready for use.
[0031] 2. CM separation:
[0032] Centrifuge: Beckman Avanti J-E high-speed refrigerated centrifuge;
[0033] Rotor, centrifuge tube: JA-20: 20000rpm, 18400×g, 8×50ml;
[0034] Centrifugation parameters: 4℃, 20000rpm×2h;
[0035] Centrifugation result: CM is white and floats on the surface of the liquid, or close to the wall of the centrifuge tube, carefully suck out.
[0036] 3. Separation of plasma lipoproteins:
[0037] Centrifuge: Hitachi CP-80WX ultra-speed centrifuge;
[0038] Rotor, centrifuge tube: P70AT: 70000rpm, 505000×g, 8×40ml, 40PA tube.
[0039] 3.1, VLDL separation:
[0040] Plasma treatment: mix the plasma after removing CM, add ultrapure water or ...
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