Cannabis seed soft pseudo-carbonization fermented granules and preparation method and application thereof
A technology of hemp seeds and granules is applied in the fields of biochemical equipment and methods, fermentation, microorganism-based methods, etc., and can solve the problems of insignificant adsorption effect of adsorption material particles, poor, inability to directly incense cigarette aroma coordination, etc. To achieve good application value, improve the effect of increasing aroma and reducing coke, and enriching the aroma of cigarettes
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Embodiment 1
[0035] 1. Soft carbonization of hempseed
[0036] Put the hemp seeds into the carbonization furnace, gradually raise the furnace temperature to about 80°C, and keep it for 40 minutes. After the hemp seeds are dry, quickly raise the temperature to 120°C, and keep it for 3 hours. Generally, the raw material turns taupe, and the carbonization is completed when the weight is 50-80% of that before the carbonization.
[0037] 2. Cultivation of Bacillus subtilis Van3 (B.subtilisVan3)
[0038] A. Test tube slant culture
[0039]A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, incubate at 28°C for 24 hours to obtain test tube seeds, transfer the liquid strains to 1L of LB liquid medium according to the inoculation amount of 5% (volume percentage of liquid strains, v / v), shake at 37...
Embodiment 2
[0045] 1. Soft carbonization of hempseed
[0046] Put the hemp seeds into the carbonization furnace, gradually raise the furnace temperature to 80°C, and keep it for 30 minutes. After the hemp seeds are dry, quickly raise the temperature to 140°C, and keep it for 2.5 hours.
[0047] 2. Cultivation of Bacillus subtilis Van3
[0048] B. Seed culture
[0049] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate Van35 test tube species, cultivate at 28°C for 24h to obtain liquid strains, transfer the liquid strains to 1L of LB liquid medium at 5% (volume percentage of liquid strains, v / v), 37°C, 150r Centrifuge the culture solution at 3500 r / min for 10 min, wash the precipitate with sterile water, centrifuge, and finally shake evenly with 20 mL of sterile water, and dilute it 10 times before use, which is the Bacillus sub...
Embodiment 3
[0054] Example 3: Soft pseudo-carbonized fermented granules of hempseeds applied to cigarette filter sticks
[0055] The soft quasi-carbonized fermented granules of hemp seed obtained in Example 1 are applied to cigarette composite cigarette filter sticks, and 1.5 mg of granular material is added to each millimeter of standard cigarette filter sticks to prepare experimental cigarettes, which are named as No. 1 cigarettes; Cigarettes without the above-mentioned particles were used as the control sample, and the moisture content of the cigarette samples was adjusted to 12wt% according to the national standard "GB / T16447-1996 Atmospheric Environment for the Regulation and Testing of Tobacco and Tobacco Products", and the smoking expert group was evaluated according to "GB5606.4- 1996 Cigarette Sensory Technical Requirements" to carry out sensory evaluation of cigarettes and detection of harmful components in smoke. See Table 1 for the evaluation results; see Table 2 and Table 3 f...
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