A plant soft pseudo-carbonization fermentation granule, its preparation method and application
A technology of plants and granules, applied in biochemical equipment and methods, methods based on microorganisms, fermentation, etc., can solve the problems of insignificant adsorption effect, poor adsorption material particles, poor, and inability to directly flavor cigarette aroma coordination, etc., to achieve Good application value, convenient operation, and the effect of enriching the aroma of cigarettes
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Embodiment 1
[0036] 1. Soft carbonization of Ganoderma lucidum
[0037] Put the Ganoderma lucidum into the carbonization furnace, gradually raise the furnace temperature to about 80°C, and keep it for 40 minutes. After the Ganoderma lucidum is dry, quickly raise the temperature to 120°C, and keep it for 3 hours. Generally, the raw material turns taupe, and the carbonization is completed when the weight is 50-80% of that before the carbonization.
[0038] 2. Cultivation of Bacillus subtilis Van3 (B.subtilisVan3)
[0039] A. Test tube slant culture
[0040]Adopt Luria-Bertani (LB) medium, formula is: peptone 10g, sodium chloride 10g, yeast extract 5g, agar 20g, distilled water is settled to 1000ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, incubate at 28°C for 24 hours to obtain test tube seeds, transfer the liquid strains to 1L of LB liquid medium according to the inoculation amount of 5% (volume percentage of liquid strains, v / v), shake at 37°C, 150r / min ...
Embodiment 2
[0045] Example 2: Ganoderma lucidum soft pseudo-carbonized fermented granules applied to cigarette filter sticks
[0046] Apply the Ganoderma lucidum softening carbonization fermentation granules obtained in Example 1 to the composite cigarette filter sticks of cigarettes, add 1.5 mg of granular material per millimeter of standard cigarette filter sticks, and prepare experimental cigarettes, which are named as No. 1 cigarettes; The cigarettes with the above-mentioned particles were used as the control sample, and the moisture content of the cigarette samples was adjusted to 12wt% according to the national standard "GB / T16447-1996 Atmospheric Environment for Regulation and Testing of Tobacco and Tobacco Products", and the smoking expert group was tested according to "GB5606.4-1996 Tobacco Sensory Technical Requirements” is used to carry out sensory evaluation of cigarettes and detection of harmful components in smoke. See Table 1 for the evaluation results; see Table 2 and Tabl...
Embodiment 3-9
[0047] Example 3-9: Preparation of other plant soft carbonization fermentation granules
[0048] Same as Example 1, the difference is that the Ganoderma lucidum is replaced by pine cones, coffee, maca, hemp seeds, cinnamon bark, tree moss or small green oranges respectively. Others were the same as in Example 1, and the plant soft carbonized fermentation granules with a moisture content of 12 wt%, a rough surface, and a particle size of 40 mesh were respectively obtained.
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