Ganoderma lucidum soft carbonized fermentation granule, preparation method and use thereof
A technology of granules and Ganoderma lucidum, which is applied in the field of cigarette tip sticks, its preparation, and Ganoderma lucidum soft pseudo-carbonization fermentation granules, can solve the problems of insignificant adsorption effect of adsorption material particles, inability to directly flavor cigarettes, poor coordination of aroma, etc., to achieve Good application value, rich cigarette flavor, and convenient operation
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Embodiment 1
[0035] 1. Soft carbonization of Ganoderma lucidum
[0036] Put the Ganoderma lucidum into the carbonization furnace, gradually raise the furnace temperature to about 80°C, and keep it for 40 minutes. After the Ganoderma lucidum is dry, quickly raise the temperature to 120°C, and keep it for 3 hours. Generally, the raw material turns taupe, and the carbonization is completed when the weight is 50-80% of that before the carbonization.
[0037] 2. Cultivation of Bacillus subtilis Van3 (B.subtilisVan3)
[0038] A. Test tube slant culture
[0039]A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, incubate at 28°C for 24 hours to obtain test tube seeds, transfer the liquid strains to 1L of LB liquid medium according to the inoculation amount of 5% (volume percentage of liquid stra...
Embodiment 2
[0045] 1. Soft carbonization of Ganoderma lucidum
[0046] Put the Ganoderma lucidum into the carbonization furnace, gradually raise the furnace temperature to 80°C, and keep it for 30 minutes. After the Ganoderma lucidum is dry, quickly raise the temperature to 140°C, and keep it for 2.5 hours.
[0047] 2. Cultivation of Bacillus subtilis Van3
[0048] B. Seed culture
[0049] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate Van35 test tube species, cultivate at 28°C for 24h to obtain liquid strains, transfer the liquid strains to 1L of LB liquid medium at 5% (volume percentage of liquid strains, v / v), 37°C, 150r Centrifuge the culture solution at 3500 r / min for 10 min, wash the precipitate with sterile water, centrifuge, and finally shake evenly with 20 mL of sterile water, and dilute it 10 times before use, whi...
Embodiment 3
[0054] Example 3: Ganoderma lucidum soft carbonization fermented granules applied in cigarette filter sticks
[0055] The Ganoderma lucidum softening carbonization fermentation granules obtained in Example 1 are applied to cigarette composite cigarette filter sticks, and 1.5 mg of granular material is added per millimeter of standard cigarette filter sticks to prepare experimental cigarettes, which are named as No. 1 cigarettes; The cigarettes with the above-mentioned particles were used as the control sample, and the moisture content of the cigarette samples was adjusted to 12wt% according to the national standard "GB / T16447-1996 Atmospheric Environment for Regulation and Testing of Tobacco and Tobacco Products", and the smoking expert group was tested according to "GB5606.4-1996 Tobacco Sensory Technical Requirements” is used to carry out sensory evaluation of cigarettes and detection of harmful components in smoke. See Table 1 for the evaluation results; see Table 2 and Tab...
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