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Application of rheumatic pain-relieving traditional Chinese medicinal composition to preparation of anti-angiogenesis medicament

An anti-vascular and composition technology, applied in the field of pharmaceutical use in the preparation of anti-angiogenesis drugs, can solve the problems of unclear and reduced effects

Active Publication Date: 2019-03-01
TONGHUA GOLDEN-HORSE PHARM IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have proved that Fengshi Qutong Capsule has anti-inflammatory and analgesic effects, inhibits the activation of macrophages, reduces the content of IL-1β, IL-6, and TNF-α in the serum of rheumatoid arthritis, and improves the body's scavenging of oxygen free radicals It can effectively treat rheumatoid arthritis, but its effect on tumors, psoriasis and other angiogenesis diseases is still unclear

Method used

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  • Application of rheumatic pain-relieving traditional Chinese medicinal composition to preparation of anti-angiogenesis medicament
  • Application of rheumatic pain-relieving traditional Chinese medicinal composition to preparation of anti-angiogenesis medicament
  • Application of rheumatic pain-relieving traditional Chinese medicinal composition to preparation of anti-angiogenesis medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 rheumatic pain dispelling composition

[0019] ① Raw materials: Cortex Phellodendri 78.0g, Atractylodes 78.3g, Clematis 58.6g, Caulis Spatholobus 78.0g, Honeycomb 38.8g, Snake 58.0g, Snake 27.0g, Viper 18.7g, Safflower 11.6g, 7.5g ground beetle, 18.8g myrrh (fried), 18.8g frankincense (fried), 19.6g eucalyptus, 18.7g scorpion, 11.5g centipede, 20.0g earthworm, 19.6g notopterygium, 10.0g cassia twig, 12.0g turmeric ;

[0020] ② Take Atractylodes atractylodis and Clematis and add water to moisten them. After they are thoroughly moistened, steam them and distill them for 6 hours to extract volatile oil. Turmeric, lovage, and notopterygium are moistened with water. After soaking thoroughly, steam and distill for 6 hours to extract volatile oil.

[0021] ③The dregs of Atractylodes atractylodes and Clematis clematis and safflower are decocted three times with water, the amount of water added is 10, 8, and 8 times in sequence, and the time is ...

Embodiment 2

[0029] Therapeutic effect of embodiment 2 rheumatism dispelling pain traditional Chinese medicine composition to rat thoracic aortic ring angiogenesis

[0030] 1. Experimental animals and materials

[0031] SD rats, male, 180-200g, 5, purchased from China National Institutes for Food and Drug Control, license: SCXK (Beijing) 2014-0013. Rheumatic pain dispelling traditional Chinese medicine composition is prepared by embodiment 1.

[0032] 2. Establishment and administration of rat arterial ring experimental model

[0033] A spare set of high-pressure surgical instruments in advance. Mix equal amounts of Matrigel and H-DMEM, coat a 48-well plate, 100 μL / well, and incubate in a constant temperature incubator at 37°C for 30 min to solidify the Matrigel; sacrifice SD rats by cervical dislocation, and dissolve in 75% ethanol Disinfect for 10 minutes; open the rat chest cavity, peel off the rat thoracic aorta, take it out and put it in PBS containing double antibodies, wash the b...

experiment example 3

[0036] Experimental Example 3 Inhibition of Rheumatism Qutong Traditional Chinese Medicine Composition on HUVEC Cell Proliferation, Migration, Adhesion, Invasion and Tube Formation

[0037] 1. Materials

[0038] HUVEC was purchased from Shanghai Zishi Biotechnology Co., Ltd., and 4-8 generations were used for experiments. Rheumatic pain dispelling traditional Chinese medicine composition is prepared by embodiment 1.

[0039] 2. Experimental method

[0040] 2.1 Cell cryopreservation

[0041] Cells were frozen in order to maintain a similar cell generation number during the experiment. When the degree of fusion reaches about 80% and the cells are in the logarithmic growth phase, the cells can be frozen. The specific steps for freezing the cells are as follows:

[0042] (1) The culture dish for culturing cells was taken out horizontally from the incubator, and placed under a microscope to observe the growth morphology and fusion degree of the cells. When the cells are in the...

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Abstract

The invention belongs to the technical field of medicine, and relates to application of a rheumatic pain-relieving traditional Chinese medicinal composition to preparation of an anti-angiogenesis medicament. As proved by human umbilical vein endothelial cell (HUVEC) tube formation assay, MTT, Transwell migration and invasion as well as adhesion experiments, rat aorta ring angiogenesis experiments,and tumor angiogenesis experiments, the rheumatic pain-relieving traditional Chinese medicinal composition can remarkably inhibitthe proliferation, migration, invasion, adhesion and tube forming capability of endothelial cells, and remarkably inhibit the generation of rat aorta ring blood vessels and implanted tumor blood vessels, indicating that the rheumatic pain-relieving traditional Chinese medicinal composition can be applied to the preparation of ananti-angiogenesis medicament.

Description

technical field [0001] The application relates to a new pharmaceutical application of a traditional Chinese medicine composition for rheumatic pain dispelling, especially the pharmaceutical application in the preparation of anti-angiogenesis drugs. Background technique [0002] Angiogenesis refers to the development of new blood vessels in the original capillaries or subsequent veins. In the process of angiogenesis, endothelial cells play a vital role. Firstly, vasodilation increases the permeability, and the macromolecular plasma proteins such as fibrin leak out; at the same time, vascular smooth muscle cells separate, the stability of the vascular wall decreases, and the vascular basement membrane is degraded. In this case, proliferative endothelial cells migrate to areas of low vascular density according to the concentration gradient of chemokines. Endothelial cells gradually gather to form vascular cords, while mesenchymal cells surround endothelial cells to proliferate...

Claims

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Application Information

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IPC IPC(8): A61K36/9066A61P9/10A61P17/06A61P27/02A61P35/00A61K35/583A61K35/62A61K35/64A61K35/646A61K35/648
CPCA61K35/583A61K35/62A61K35/64A61K35/646A61K35/648A61K36/232A61K36/237A61K36/284A61K36/286A61K36/324A61K36/328A61K36/486A61K36/54A61K36/716A61K36/756A61K36/9066A61P9/10A61P17/06A61P27/02A61P35/00A61K2300/00
Inventor 荆宇刘春芳林娜苗艳东何莲花
Owner TONGHUA GOLDEN-HORSE PHARM IND CO LTD
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