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Fowl pox heat-resistant freeze-drying protectant and its application

A heat-resistant protective agent and heat-resistant freeze-drying technology, which can be used in freeze-dried transportation, veterinary vaccines, antiviral agents, etc., can solve the problems of shortened freeze-drying time, long freeze-drying time, complex formula, etc. Less dosage, simple formula, good safety effect

Active Publication Date: 2021-04-23
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the shortcomings of the above processes such as complex formula and long freeze-drying time, the formula and freeze-drying curve of heat-resistant freeze-drying protective agent are improved to make the formula simpler and shorten the freeze-drying time, which not only saves resources, but also is more suitable for large-scale production

Method used

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  • Fowl pox heat-resistant freeze-drying protectant and its application
  • Fowl pox heat-resistant freeze-drying protectant and its application
  • Fowl pox heat-resistant freeze-drying protectant and its application

Examples

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Effect test

Embodiment 1

[0028] Example 1 Screening of heat-resistant lyoprotectant for fowl pox and optimization of lyophilization curve

[0029] 1. Preparation of fowl pox heat-resistant freeze-drying protectant

[0030] Preparation of liquid A: PVP (K30) 5%, trehalose 3%, mannitol 1%, polypeptone 4%, four protective agents are mixed evenly, and then autoclaved for later use (sterilized at 116 degrees for 25 minutes).

[0031] Preparation of liquid B: mix the three protective agents of Medium199 0.3%, vitamin C (Vc) 0.3%, and glycine 0.2%, and then sterilize at 0.22um, then set aside.

[0032] In the preparation of liquid A or liquid B, the stated % is in g / ml, and the rest of the ingredients are purified water.

[0033] Mix the above liquids A and B at a ratio of 1:1 (volume ratio) to obtain a heat-resistant lyoprotectant for fowl pox.

[0034] The fowlpox heat-resistant freeze-drying protective agent and the fowlpox virus liquid are used to prepare a vaccine in a ratio (volume ratio) of 1:1, and...

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Abstract

The invention discloses a heat-resistant freeze-drying protective agent for chicken pox and an application thereof, and belongs to the field of preparation and application of the heat-resistant freeze-drying protective agent. The invention firstly discloses a heat-resistant freeze-drying protective agent for chicken pox, which comprises the following components: trehalose, mannitol, polypeptone and purified water. The chicken pox heat-resistant freeze-drying protective agent of the invention has a simple formula, effectively saves resources, and is more suitable for large-scale production. The invention further discloses the application of the chicken pox heat-resistant freeze-drying protective agent in preparing the chicken pox heat-resistant protective agent live vaccine. By optimizing the freeze-drying curve in vaccine preparation, the invention shortens the freeze-drying time to 24 hours, and has high vaccine preparation efficiency. The fowl pox heat-resistant protective agent live vaccine prepared by the invention not only has high efficacy, but also can be stored for 36 months under the condition of 2-8° C., the titer decreases less after heat-resistant, and the efficacy of the vaccine is more stable.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant for fowl pox, and also relates to the application of the heat-resistant freeze-dry protectant for fowl pox in the preparation of a live vaccine of the heat-resistant freeze-dry protectant for fowl pox, belonging to the heat-resistant freeze-dry protectant for fowl pox Preparation and application fields. Background technique [0002] China's research on heat-resistant freeze-drying protective agents started relatively late, but now the research in this area has achieved certain results, and a heat-resistant freeze-drying protective agent for chickenpox has been developed. Parts are usually: gelatin 3%, lactose 5-6%, EDTA 2%, PVP (K90) 5%, sorbitol 2%, sodium glutamate 1%, beef peptone 2%, bovine serum albumin 1%, Vc 0.1%; the preparation is the dry matter component, and the rest of the percentage is distilled water. The effective components of the autoclavable and high-temperature-resi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/26A61K47/42A61K39/275A61P31/20
CPCA61K9/19A61K39/12A61K47/26A61K47/42A61K2039/5254A61K2039/552A61P31/20C12N2710/24034
Inventor 孙德君步帆柴华梁宛楠窦海艳冯艳男王宁杨大伟李琳毕德峰
Owner 哈药集团生物疫苗有限公司
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