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A kind of modification method of agarose

A modification method and agarose technology are applied in the field of agarose modification and can solve the problems of weakening the hydrogen bond between sugar chains, reducing the phase transition temperature of agarose, affecting drug absorption and the like.

Active Publication Date: 2021-04-27
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods of modifying agarose are mainly by introducing small molecular functional groups into the agarose structure, weakening the hydrogen bond interaction between sugar chains in the gelation process, and reducing the phase transition temperature of agarose; the agarose produced by these modification methods In clinical application, there will be technical defects that have an immune reaction with the human body and affect the absorption of drugs by the human body

Method used

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  • A kind of modification method of agarose
  • A kind of modification method of agarose
  • A kind of modification method of agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A modification method of agarose, the method is:

[0041] Add 0.1 g of low-melting point agarose and 100 mL of deionized water into a round-bottomed flask, and heat to fully dissolve the agarose. Cool to 50°C, add 0.5g sodium hydroxide (NaOH) and 1.6mL epichlorohydrin, react at 30°C for 2h, add 5mL containing 0.025g methoxy polyethylene glycol amine (mPEG-NH 2 ) (molecular weight is 20k) deionized water, wherein the mass ratio of agarose to methoxypolyethylene glycol amine is 1:0.25; after reacting at 30°C for 72h, the reaction solution is transferred to a dialysis bag, and the retention of the dialysis bag The molecular weight is 50k, dialyzed for 3 days, changing the water every 12 hours, and freeze-dried. The polymer prepared in this embodiment is: Agarose-g-PEG 20k -1.

Embodiment 2

[0043] Other experimental parameters of this embodiment are the same as in Example 1, except that methoxypolyethylene glycol amine (mPEG-NH 2 ) content is 0.05g; the mass ratio of agarose to methoxypolyethylene glycol amine is 1:0.5. The polymer prepared in this embodiment is: Agarose-g-PEG 20k -2.

Embodiment 3

[0045] Other experimental parameters of this embodiment are the same as in Example 1, except that methoxypolyethylene glycol amine (mPEG-NH 2 ) content is 0.1g; the mass ratio of agarose to methoxypolyethylene glycol amine is 1:1. The polymer prepared in this embodiment is: Agarose-g-PEG 20k -3.

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Abstract

The present invention relates to the technical field of agarose, in particular to a method for modifying agarose. In the present application, sodium hydroxide and epichlorohydrin are added to an aqueous solution of agarose with a low melting point, reacted at 30°C for 2 hours, and different masses of formazan are added. Oxypolyethylene glycol amine was reacted at 30°C for 72 hours, and after dialysis and freeze-drying, agarose-grafted-polyethylene glycol monomethyl ether polymer (Agarose-g-mPEG) was prepared. The modification method can introduce a hydrophilic macromolecule segment PEG into the agarose structure, destroy the formation of the agarose double helix structure, and reduce the phase transition temperature of the agarose.

Description

【Technical field】 [0001] The invention relates to the technical field of agarose, in particular to a method for modifying agarose. 【Background technique】 [0002] Agarose is a chain-like neutral polysaccharide composed of D-galactose and 3,6-intraether-L-galactose. The structural unit contains hydroxyl functional groups, which are easy to form hydrogen with the hydrogen atoms in the structural unit and the water molecules around the chain segment. key. At low temperature, the sugar chains of agarose rely on hydrogen bonds to intertwine with each other in a double helix, forming a double helix structure, closely arranged, forming a gel, and appearing white and turbid; at high temperatures, the hydrogen bonds in the structural units are destroyed, resulting in The random coils are dispersed in water, the solution is clear and transparent, and it is a kind of polymer with special temperature response. [0003] Agarose isolated in nature has a high phase transition temperature...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/12
CPCC08B37/0039
Inventor 黄岗唐培朵黄华林卢波陈东孙靓关妮陆琦
Owner GUANGXI ACAD OF SCI
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