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Detecting method for measuring coagulation accelerator effect by applying blood coagulation way

A blood coagulation and detection method technology, applied in the preparation of test samples, biological testing, material inspection products, etc., can solve the problems of coagulant inactivation, coagulant effect decline, coagulant formula confusion, etc., to achieve Calcium ion concentration is reduced, blood coagulation is not easy, and the effect of observing the reaction result is good

Inactive Publication Date: 2019-03-19
福州长庚医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, as far as the current domestic manufacturers of blood collection devices are concerned, the coagulant formula used is very confusing
To sum up, there are no more than two types of biological type and non-polar type, or a combination of the two types. The level of such coagulant accelerators is uneven; in addition, because coagulant accelerators are easy to lose activity under the influence of the environment, the two types Either situation will lead to a decrease in the effectiveness of the coagulant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A detection method for measuring the effect of a coagulant by using a blood coagulation method, comprising the following steps:

[0033] 1) Preliminary preparation: select a room temperature of 25°C for the detection test;

[0034] Prepare a 100~1000μL pipette and matching suction tip; prepare a 10~100uL pipette and matching suction tip;

[0035] 2) Draw blood samples: according to the amount of plasma, quantitatively draw blood to the nominal capacity of the blood collection bag of 400mL;

[0036] 3) Blood sample processing: Let the blood drawn in step 2) stand still for 30 minutes and then centrifuge. The centrifugal force is set between 1600g and the centrifugation time is controlled to 10 minutes;

[0037] When detecting diatomaceous earth-based inorganic coagulants: after step 3), go to step 4):

[0038] 4) Mixing and fusion: extract 200uL of plasma in step 3) and add it to an anticoagulant tube with diatomaceous earth inorganic coagulant and sodium citrate 1:9, ...

Embodiment 2

[0048] A detection method for measuring the effect of a coagulant by means of blood coagulation, characterized in that it comprises the following steps:

[0049] 1) Preliminary preparation: select a room temperature of 20°C for the detection test;

[0050] Prepare a 100~1000μL pipette and matching suction tip; prepare a 10~100uL pipette and matching suction tip;

[0051] 2) Draw blood samples: according to the amount of plasma, quantitatively draw blood to the nominal capacity of the blood collection bag of 400mL;

[0052] 3) Blood sample processing: Let the blood drawn in step 2) stand for 40 minutes and then centrifuge, the centrifugal force is set between 2000g, and the centrifugation time is controlled at 5 minutes;

[0053] When detecting diatomaceous earth-based inorganic coagulants: after step 3), go to step 4):

[0054] 4) Mixing and fusion: extract 200uL of plasma in step 3) and add it to an anticoagulant tube with diatomaceous earth inorganic coagulant and sodium cit...

Embodiment 3

[0064] A detection method for measuring the effect of a coagulant by using a blood coagulation method, comprising the following steps:

[0065] 1) Preliminary preparation: select a room temperature of 23°C for the detection test;

[0066] Prepare a 100~1000μL pipette and matching suction tip; prepare a 10~100uL pipette and matching suction tip;

[0067] 2) Draw blood samples: according to the amount of plasma, quantitatively draw blood to the nominal capacity of the blood collection bag of 400mL;

[0068] 3) Blood sample processing: Let the blood drawn in step 2) stand for 45 minutes and then centrifuge. The centrifugal force is set at 1800g, and the centrifugation time is controlled at 7 minutes;

[0069] When detecting diatomaceous earth-based inorganic coagulants: after step 3), go to step 4):

[0070] 4) Mixing and fusion: extract 200uL of plasma in step 3) and add it to an anticoagulant tube with diatomaceous earth inorganic coagulant and sodium citrate 1:9, shake the a...

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PUM

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Abstract

The invention discloses a detecting method for measuring a coagulation accelerator effect by applying a blood coagulation way and relates to the technical field of coagulation accelerators. The methodcomprises the following steps: early-stage preparation, blood sample extraction, blood sample treatment, uniform mixing and melting, multiple testing and result determination. The effects of a diatomite inorganic coagulation accelerator and a thrombase biological coagulation accelerator are reversely tested by controlling a condition such as the dosage of added calcium and plasma, so that a coagulation accelerator with a high blood coagulation effect is conveniently screened, and furthermore, the coagulation accelerator is utilized in medical examination work at higher detecting efficiency within shorter time. By using the screened coagulation accelerator, the checking time is shortened, and the detecting efficiency is increased, so that the market demand is met, and the detecting efficiency of working staff in a hospital is increased.

Description

technical field [0001] The invention relates to the technical field of blood coagulation accelerators, in particular to a detection method for measuring the effect of coagulation accelerators by means of blood coagulation. Background technique [0002] The speed of medical testing has clinical significance. Some tests require serum to be separated from blood. It generally takes one hour for blood to coagulate to precipitate serum, and it takes about half an hour to warm and centrifuge in time, and it is easy to cause hemolysis. If there is an emergency cross matching or emergency diagnosis, the detection time will be prolonged, and even the treatment of the disease will be delayed. , so how to shorten the time of serum separation is the key to the inspection work. [0003] Blood coagulation accelerator is the core raw material for the production of coagulation accelerator tubes. The application amount is large, the technical requirements are high, the imported products are...

Claims

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Application Information

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IPC IPC(8): G01N33/86G01N1/38
CPCG01N1/38G01N33/86
Inventor 钟德优沈玉建黄丽芳
Owner 福州长庚医疗器械有限公司
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