Unlock instant, AI-driven research and patent intelligence for your innovation.

Uses of kdm4d

A DMP1, gene technology, applied in the field of KDM4D, can solve the problems of unclear molecular mechanism, limiting the potential application of mesenchymal stem cells, etc.

Active Publication Date: 2021-03-26
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After finding possible seed cells, the molecular mechanism of directed differentiation of mesenchymal stem cells remains unclear, which also limits the potential application of mesenchymal stem cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uses of kdm4d
  • Uses of kdm4d
  • Uses of kdm4d

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Cell culture:

[0039] All stem cells involved in the present invention comply with the behavioral guidelines for human embryonic stem cell research, and the use of human tissue has been approved by the Ethics Committee of Capital Medical University, and all volunteers signed an informed consent before the operation; wisdom teeth were disinfected with 75% alcohol Then wash with phosphate buffered saline, isolate and culture and identify stem cells of the apical papilla. The brief description is as follows: Gently peel off the apical papilla tissue, wash it repeatedly with phosphate buffered saline, cut it into pieces, and place it in a place containing type Ⅰ collagenase ( 3g / L) and dispase (4g / L), digested at 37°C for 1 hour, passed through a 70μm cell sieve to collect cells, centrifuged at 1000rpm for 10min, and resuspended with culture medium to form a single cell suspension; the cells were inoculated in a 25cm 2 In the cell culture flask, in the medium (containin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological medicine and especially relates to application of KDM4D. Research results show that the KDM4D disclosed by the invention can affect in-vitroand in-vivo mineralization ability of mesenchymal stem cells by regulating expression of osteogenesis and tooth formation genes, affecting differentiation of the mesenchymal stem cells to the osteogenesis and the tooth formation and affecting migration chemotaxis ability of stem cells. The application of the KDM4D disclosed by the invention can provide technical support for preparing bone tissue or tooth tissue regeneration medicine containing KDM4D genes.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to the use of KDM4D. Background technique [0002] Tooth loss is a relatively common phenomenon at present. For tooth loss, the current restoration methods mainly include denture restoration and implant restoration. However, these traditional restoration methods have various shortcomings, which makes people focus on the field of "tooth regeneration". , Tooth regeneration has opened up a new way for the restoration of clinical tooth loss, and one of the key issues restricting the study of tooth regeneration is the source of tooth regeneration seeds and the mechanism of stem cell directional differentiation. [0003] In the field of tooth regeneration research, stem cells are an important source of seed cells. Stem cells are a type of undifferentiated cells with self-replication ability and multi-differentiation potential. Dental stem cells mainly used for dental tissue engineeri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N5/077
CPCC12N5/0654C12N15/86C12N2740/15043
Inventor 范志朋张琛梁寒冰李倩杨昊清曹杨杨
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV