Establishment and selection method of recurrent selection population for high-quality multi-resistance tobacco new line breeding
A technology of reincarnation selection and new strains, which is applied in the field of reincarnation selection group creation and selection in the breeding of high-quality multi-resistant tobacco new strains, can solve the problems of reincarnation selection occupying a large area of land and heavy workload, and achieve a high degree of gene aggregation, Enhanced dosage effect and excellent properties
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Embodiment 1
[0044] Example 1: Creation and selection of flue-cured tobacco reincarnation selection groups
[0045]In order to take into account the long-term and short-term breeding goals, when selecting the parents of the original population for the construction of flue-cured tobacco reincarnation, high quality and wide adaptability should be given priority to, and the resistance to all common and major diseases of flue-cured tobacco should be considered as much as possible. According to the breeding goal, the selected parents should include quality character parents, adaptive parents, high-yield and easy-to-roast parents, and disease-resistant parents. In order to use varieties in production with wide adaptability, the high-yield and easy-to-roast parents should include broad-leaved high-yield and easy-to-roast parents and multi-leaf high-yield and easy-to-roast parents, and the disease-resistant parents should include root disease-resistant parents, foliar disease-resistant Parents a...
Embodiment 2
[0054] Example 2: The introduction of heterologous germplasm in the recurrent selection population of flue-cured tobacco
[0055] In the summer of 2014, it was found that the reincarnation selection population had deficiencies in quality traits and resistance to black shank and bacterial wilt. Therefore, in the winter of the same year, the high-quality parent K326 was used as the female parent at the Sanya Southern Breeding Base, which is also resistant to black shank and bacterial wilt. Blight germplasm DB101 was used as the male parent, and the hybrid combination K326×DB101 was prepared.
[0056]In 2015, the first round of selection group C1 seeds obtained in 2014 and the F1 generation seeds of the hybrid combination K326×DB101 were sown in the Lichuan Experimental Base to raise seedlings, and the seedlings were transplanted into the field respectively. When flowering, select 500 tobacco plants with ideal growth and morphological traits in the first-round selection populat...
Embodiment 3
[0059] Example 3: Identification of excellent individual plants of flue-cured tobacco recurrent selection population
[0060] In 2013, 500 tobacco plants selected through disease resistance identification and also resistant to wildfire, black shank, common mosaic virus, climatic spot, root-knot nematode and red spot disease will be crossed between individual plants At the same time, the F3 generation seeds of the hybrids were obtained by selfing with Liuyancha, and the seeds were harvested per plant.
[0061] In 2014, 500 F3 generation seeds were sown in individual plants in the greenhouse seedbed of the Lichuan Experimental Base, and each individual plant had 50 tobacco seedlings, which was called a strain. When the seedlings are about 8 weeks old, inoculate and identify the resistance to angular spot disease. First, select the lines with better overall resistance, and then select the tobacco seedlings resistant to angular spot disease in the selected lines. A total of 451...
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