A kind of RNA extraction method

Abstract

The invention discloses an RNA extraction method. The RNA extraction method comprises the following specific steps of (1) preserving tissues or cells with an extracting solution A, then adding an extracting solution B to the tissues or cells preserved in the extracting solution A, and performing uniform mixing so as to obtain a mixture; (2) when RNA is extracted from tissues, performing ultrasonicbreaking on the mixture; (3) when RNA is extracted from the cells, directly performing operations in the step (5); (4) reversing the mixture after ultrasonic breaking to achieve a uniform mixing state; (5) adding chloroform to the mixture obtained through extracting the RNA from the cells in the step (3) or the mixture obtained in the step (4), performing centrifugation, and taking upper-layer liquid; (6) adding isopropyl alcohol to the upper-layer liquid, and then performing centrifugation so as to obtain precipitate; (7) adding an ethanol solution to the precipitate, performing uniform mixing, performing centrifugation, and discarding liquid; and (8) finally adding water for dissolving. The method is high in rate, incomplete tissue breaking and interference of endogenous RNA enzymes areavoided, the extraction time is greatly shortened, and the extraction efficiency of the RNA is further improved.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an RNA extraction method. Background technique [0002] In modern molecular biology experiments and clinical molecular diagnosis, it is often necessary to isolate and purify RNA from tissues and cells, and the quality of RNA often affects the success or failure of molecular biology experiments such as cDNA library, RT-PCR and Northern Blot. [0003] The commonly used method is to first protect the tissue with a tissue protection solution, and then use the extraction solution to extract the RNA in the tissue or cells. Commonly used protection methods include liquid nitrogen protection and protection solution protection, and the commonly used protection solutions include RNA later reagents and RNA safety reagents. [0004] Among them, the liquid nitrogen protection method not only requires harsh conditions, but also has high costs, and is only suitable ...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of low efficiency due to the incompatibility between the existing solution preservation and the existing extraction solution caused by the existing technology, the present invention provides an RNA extraction method

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