GGT antibody immune nano magnetic beads and preparation method and application thereof

Abstract

The invention discloses GGT antibody immune nano magnetic beads and a preparation method and application thereof. The GGT antibody immune nano magnetic beads are of a core-shell structure, a core is magnetic material Fe3O4, a middle layer is high polymer liposome, and anoutermost layer is a GGT antibody. The high polymer liposome is a mixture of carboxymethyl chitosan hexadecyl quaternary ammoniumsalt and cholesterol. According to the GGT antibody immune nano magnetic beads and the preparation method and application thereof, the GGT antibody is connected to the Fe3O4 nano magnetic beads, a green fluorescent GGT antibody is combined with glioma cells in blood enriched on the surface of glioma, the subsequent detection process is the same as EpCAM kit detection, the glioma cells are determined by specifically expressing GGT protein expression on the surfaces of the glioma cells, the GGT antibody immune nano magnetic beads are clinically reasonable and practical, changes of tumor cells can be dynamically detected through CTC analysis, treatment status and disease progress of patients can be dynamically reflected in real time, and noninvasive and accurate supplement is provided for current tumor treatment monitoring.

Description

technical field

[0001] The invention relates to the technical field of immune nano magnetic beads, in particular to a GGT antibody immune nano magnetic beads and its preparation method and application. Background technique

[0002] Glioma is one of the top ten malignant tumors in the human body, and it is also the most common tumor of the central nervous system. Among them, glioblastoma multiforme GBM is the most common, and has the characteristics of strong invasion, high lethality, and easy recurrence. At present, the common problem in clinical practice is that the pseudo-progression of chemoradiotherapy reaction and the true recurrence of glioma are difficult to distinguish in some patients after the completion of radiotherapy and chemotherapy. It is a key technology for the period and a major demand for precision medicine.

[0003] Circulating tumor cells (CTCs) refer to tumor cells that are released from solid tumors or metastases into the peripheral blood circulation ...

Examples

Embodiment 1

[0041] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a high polymer liposome, and the outermost layer is GGT antibody; the high polymer liposome is a mixture of carboxymethyl chitosan cetyl quaternary ammonium salt and cholesterol.

Embodiment 2

[0043] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a polymer liposome, and the outermost layer is GGT antibody; the polymer liposome is a mixture of carboxymethyl chitosan hexadecyl quaternary ammonium salt and cholesterol; carboxymethyl chitosan Sugar hexadecyl quaternary ammonium salt and cholesterol, magnetic material Fe 3 o 4 The mass ratio to GGT antibody is 15:12:36:1.

Embodiment 3

[0045] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a polymer liposome, and the outermost layer is GGT antibody; the polymer liposome is a mixture of carboxymethyl chitosan hexadecyl quaternary ammonium salt and cholesterol; carboxymethyl chitosan Sugar hexadecyl quaternary ammonium salt and cholesterol, magnetic material Fe 3 o 4 The mass ratio to GGT antibody is 20:16:48:1.

technical field

[0001] The invention relates to the technical field of immune nano magnetic beads, in particular to a GGT antibody immune nano magnetic beads and its preparation method and application. Background technique

[0002] Glioma is one of the top ten malignant tumors in the human body, and it is also the most common tumor of the central nervous system. Among them, glioblastoma multiforme GBM is the most common, and has the characteristics of strong invasion, high lethality, and easy recurrence. At present, the common problem in clinical practice is that the pseudo-progression of chemoradiotherapy reaction and the true recurrence of glioma are difficult to distinguish in some patients after the completion of radiotherapy and chemotherapy. It is a key technology for the period and a major demand for precision medicine.

[0003] Circulating tumor cells (CTCs) refer to tumor cells that are released from solid tumors or metastases into the peripheral blood circulation ...

Examples

Embodiment 1

[0041] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a high polymer liposome, and the outermost layer is GGT antibody; the high polymer liposome is a mixture of carboxymethyl chitosan cetyl quaternary ammonium salt and cholesterol.

Embodiment 2

[0043] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a polymer liposome, and the outermost layer is GGT antibody; the polymer liposome is a mixture of carboxymethyl chitosan hexadecyl quaternary ammonium salt and cholesterol; carboxymethyl chitosan Sugar hexadecyl quaternary ammonium salt and cholesterol, magnetic material Fe 3 o 4 The mass ratio to GGT antibody is 15:12:36:1.

Embodiment 3

[0045] A kind of GGT antibody immune nano-magnetic beads, which is a core-shell structure, and the inner core is a magnetic material Fe 3 o 4 , the middle layer is a polymer liposome, and the outermost layer is GGT antibody; the polymer liposome is a mixture of carboxymethyl chitosan hexadecyl quaternary ammonium salt and cholesterol; carboxymethyl chitosan Sugar hexadecyl quaternary ammonium salt and cholesterol, magnetic material Fe 3 o 4 The mass ratio to GGT antibody is 20:16:48:1.

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