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Fatty acid esters against infections in fermentations

A technology of fermentation medium and antibacterial agent, applied in the field of inhibiting the growth of Gram-positive bacteria

Pending Publication Date: 2019-04-16
PURAC BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nothing in this reference teaches or suggests the specific efficacy of lactate against Gram-positive bacteria compared to Gram-negative bacteria

Method used

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  • Fatty acid esters against infections in fermentations
  • Fatty acid esters against infections in fermentations
  • Fatty acid esters against infections in fermentations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 : Effect of myristyl-lactate (C14-lactate) on mixed cultures of Escherichia coli and Clostridium pasteuriani

[0104] To determine whether myristyl-lactate (C14-lactate) could prevent Clostridium pasteuriani JEG2 (NCCB100154, NCCB: Netherlands Culture Collection of Bacteria, Utrecht, The Netherlands) in bioengineered Homolactic-acid producing R-lactic acid Escherichia coli TG128 (NRRL B-30962, NRRL: National Agricultural Research Service Culture Collection, National Center for Agricultural Utilization Research, Peoria, Illinois, U.S.A.) The cultures were grown and three different fermentations were set up and carried out simultaneously. These ferments are:

[0105] i) Fermentation tank 1: Escherichia coli TG128 pure culture fermentation;

[0106] ii) Fermenter 2: Escherichia coli TG128 is mixed with Clostridium pasteurianus JEG2;

[0107] iii) Fermenter 3: Escherichia coli TG128 was mixed with Clostridium pasteuriani JEG2, and 0.05% (w / v) tetradecanoyl-la...

Embodiment 2

[0118] Example 2: A mixture of decanoyl-lactate (C10-lactate) and lauryl-lactate (C12-lactate) or lauryl-lactate (C12-lactate) and myristate Effects of alkanoyl-lactate (C14-lactate) mixtures on mixed cultures of Escherichia coli and Clostridium pasteuriani

[0119] In the same experimental setup as described in Example 1, the efficacy of 0.05% (w / v) AMCET 3400E and 0.05% (w / v) AMCET 4530E in inhibiting the growth of Clostridium pasteuriani JEG2 in cultures of E. coli TG128 was tested .

[0120]The performance of Fermentor 3 with AMCET 3400E or AMCET 4530E was similar in every respect to that of Fermentor 1 inoculated with a pure culture of E. coli TG128. In addition, chemical analysis of the fermentation broth showed no impurity profile between the E. coli TG128 standard fermentation (Fermenter 1) and the E. coli / C. pasteuriani JEG2 mixed culture with AMCET 3400E or AMCET 4530E (Fermenter 3). difference. For AMCET 3400E and AMCET 4530E, the percent enantiomeric excess of...

Embodiment 3

[0121] Example 3: In vitro test of lactate against Clostridium perfringens

[0122] Lactate as defined in Formula 1 and glycerides as defined in Formula 2 were tested for growth inhibitory efficacy against Clostridium perfringens ATCC 13124 in a Bioscreen C culture system.

[0123] The optical density of the culture was automatically measured at regular intervals using a broadband filter at 420-580 nm. The growth rate of the test organisms was determined at 30°C. To ensure hypoxic conditions, the Bioscreen was placed in an oxygen sensor equipped with an M-12 type (In Vivo 2 400 hypoxic workstation, in the anaerobic cabinet of Biotrace International Plc, Bridgend, United Kingdom). The oxygen tension was adjusted to 0% oxygen using a Ruskinn gas mixer module (Biotrace International Plc).

[0124] Brain heart infusion broths were prepared with varying amounts of different lactates and glycerides as shown in Table 2 below.

[0125] The following compounds were tested: capry...

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Abstract

The invention relates to the use of an antibacterial agent for suppressing the growth of gram-positive contaminating bacteria in culturing gram-negative bacteria or moulds or yeasts. The antibacterialagent is selected from: i) a lactylate in accordance with the general formula (R-(O-CH(CH3)-CO)a O)b M (Formula 1); a glycerol ester in accordance with the general formula CH2OR1-CHOR2-CH2OR3 (Formula 2); and, mixtures thereof. In said general formulae: R represents a C4-C18 acyl group, the acyl group having an alkyl or alkenyl chain which may be branched or unbranched; R1, R2 and R3 are each independently selected from H or a C4-C18 acyl group, the acyl group having an alkyl or alkenyl chain which may be branched or unbranched, with the proviso that at least one of R1, R2 or R3 is H and at least one of R1, R2, or R3 is an acyl group; M represents a proton (H+) or a counter-cation chosen from the group Li, Na, K, Ca, Mg, Zn, Fe(ll), Cu, Mn, Ag, ammonium or substituted ammonium having oneor more (C1-4)alkyl optionally substituted with one or more hydroxy; a is an integer of from 1 to 3; and, b is 1 or 2 equaling the valency of M.

Description

technical field [0001] The present invention relates to methods for inhibiting the growth of Gram-positive bacteria in the culture of Gram-negative bacteria, molds and yeasts. In particular, the present invention relates to antimicrobial agents comprising lactylate, glycerides or mixtures thereof, and the use of these antimicrobial agents in fermenter cultures. Background technique [0002] The risk of infection by other microorganisms is one of the problems common to all microbial processes using pure cultures. This hazard applies especially to large industrial fermentation processes where, due to the scale of the process, there is a greater risk of contamination by other microorganisms than in laboratory fermenter processes. [0003] In yeast fermentation, workers attempt to reduce the risk of bacterial contamination by using bacterial control agents. These agents include for example (natural) antibiotics and sulfites in concentrations which do not affect the viability o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20Y02E50/10C12N1/14C12P7/18C12P7/46C12P7/52C12P7/56C12P17/04C12N2500/36C12N1/16C12P7/06C12P7/44
Inventor R·奥托A·M·拉米雷兹J·埃尔德林克
Owner PURAC BIOCHEM