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Episomal vector for expressing foreign genes induced by tetracycline and construction method thereof

A technology of induced expression and exogenous genes, applied in the field of genetic engineering, can solve the problems of low transfection efficiency and high cost, and achieve the effect of enhancing transfection efficiency, extensive medical and research value, and expanding the ability of gene manipulation

Active Publication Date: 2021-02-02
湖北汇智铭传生物科技股份有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide an episomal vector that induces expression of exogenous genes by tetracycline, which is used to solve the technical problems of high-efficiency expression of exogenous genes in human pluripotent stem cells, low transfection efficiency and high cost in related technologies

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  • Episomal vector for expressing foreign genes induced by tetracycline and construction method thereof
  • Episomal vector for expressing foreign genes induced by tetracycline and construction method thereof
  • Episomal vector for expressing foreign genes induced by tetracycline and construction method thereof

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Embodiment Construction

[0094] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0095] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relationship between the components in a certain posture (as shown in the accompanying drawings). Relative positional relationship, movement conditions, etc., if the specific posture changes, the directional indication will also change accordingly.

[0096] In addition, in t...

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Abstract

The invention discloses a free vector for inducing and expressing exogenous genes by tetracycline. The episomal vector that induces expression of foreign genes by tetracycline includes AmpR gene fragments, ori gene fragments, SV40 poly(A) gene fragments, tight TRE promoter gene fragments, mCherry gene fragments, bGH poly(A) gene fragments, OriP gene fragment, EF-1α core promoter gene fragment, EBNA1 gene fragment, T2A gene fragment, rtTA-Advanced gene fragment, EGFP gene fragment, NeoR gene fragment and rabbitβ-globin poly(A) gene fragment. The free vector provided by the invention efficiently expresses foreign genes in human pluripotent stem cells, and has high transfection efficiency and low cost.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a free vector for inducing and expressing foreign genes by tetracycline and a construction method thereof. Background technique [0002] Genomic manipulation of human pluripotent stem cells has far-reaching scientific and medical significance. This relies on our ability to efficiently express exogenous genes in human pluripotent stem cells. Due to the nature of human pluripotent stem cells, their transfection is often inefficient or complex and expensive. Therefore, the most commonly used method at present is to randomly insert foreign genes into the cell genome through lentiviruses. However, this method may lead to uncontrollable genomic mutations in the engineered cells that form tumor cells and lose their medical value. [0003] Therefore, it is necessary to provide a new episomal vector for the expression of foreign genes induced by tetracycline and its constru...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85
CPCC12N15/85C12N2800/107C12N2830/003
Inventor 梁楠张富强郝好杰陈惠华唐成王双莹凡素琴黄霞
Owner 湖北汇智铭传生物科技股份有限公司
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