Single-chain antibody targeting CD19, chimeric antigen receptor T cell, preparation method and application thereof

A chimeric antigen receptor and single-chain antibody technology, applied in the field of medical biology, can solve the problem of failing to kill tumor cells, and achieve the effects of promoting amplification, high infection efficiency, and lasting in vivo maintenance ability.

Inactive Publication Date: 2019-06-04
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The in vivo maintenance ability (persistence) of CAR-T cells is the guarantee of the long-term efficacy of CAR-T, but in fact, CAR-T completely disappears after a few days to a few weeks after reinfusion, and fails to exert long-lasting and durable tumor killing cell capacity

Method used

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  • Single-chain antibody targeting CD19, chimeric antigen receptor T cell, preparation method and application thereof
  • Single-chain antibody targeting CD19, chimeric antigen receptor T cell, preparation method and application thereof
  • Single-chain antibody targeting CD19, chimeric antigen receptor T cell, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for preparing chimeric antigen receptor T cells targeting CD19, comprising the following steps:

[0078] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD19 targeting CD19

[0079]Prepare the coding genes of the signal peptide, single-chain antibody targeting CD19, CD8α hinge region, CD8 transmembrane region, 4-1BB signal region and CD3ζ signal region respectively, the coding gene sequence of the signal peptide is as SEQ ID NO: 15 As shown, the coding gene sequence of the single-chain antibody targeting CD19 is shown in SEQ ID NO: 2, the coding gene sequence of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene sequence of the CD8 transmembrane region The gene sequence is shown in SEQ ID NO: 9, the coding gene sequence of the 4-1BB signal region is shown in SEQ ID NO: 11, and the coding gene sequence of the CD3ζ signal region is shown in SEQ ID NO: 13.

[0080] The coding gene sequences of the above-mentioned signal peptide, si...

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PUM

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Abstract

The present invention provides a single-chain antibody targeting CD19, the single-chain antibody targeting CD19 comprises an amino acid sequence shown in SEQ ID NO: 1, and the single-chain antibody targeting CD19 is a humanized single-chain antibody. The present invention also provides a chimeric antigen receptor T cell comprising the single-chain antibody targeting CD19, wherein the chimeric antigen receptor targeting CD19 can specifically target CD19 on tumor cells, the expansion of T cells in patients is promoted, and the tumor cells can be efficiently and specifically killed. In addition,the chimeric antigen receptor T cell targeting CD19 produced by humanized single-chain antibody has better cell viability and lethality. The invention also provides a preparation method and an application of the chimeric antigen receptor T cell targeting CD19.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting CD19, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] CAR-T (Chimeric Antigen Receptor T Cell) technology is a new type of cell therapy, which is to reinfuse modified T cells into the human body, activate the autoimmune system, and carry out targeted killing of tumor cells, which can overcome the traditional The drug resistance and relapse defects of the treatment means are considered to be one of the most effective treatments for malignant tumors at present. At present, CAR-T technology has achieved remarkable results in acute and chronic lymphocytic leukemia, lymphoma and other hematological malignancies (such as B-cell lymphoma, acute and chronic B-lymphoblastic leukemia). [0003] The in vivo maintenance ability (persistence) of CAR-T cells is the guarantee of the long-term ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N5/10C12N15/867A61P35/00
Inventor 曾滢张长风吴咏东
Owner SHENZHEN BINDEBIOTECH CO LTD
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