Preparation method of yeast culture capable of improving yolk color, yeast culture and application thereof
A technology of yeast culture and egg yolk color, applied in the application, animal feed, animal feed and other directions, can solve the problems of high cost, harmful effects on the health of consumers, and increase the cost of laying hens, so as to facilitate absorption and promote animal health. , the effect of improving immunity
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Embodiment 1
[0032] As an embodiment of the present invention, the preparation method of yeast culture comprises the following steps:
[0033] 1) Preparation of liquid strains in Erlenmeyer flask
[0034] The activated Rhodotorula strains were inoculated into the seed liquid culture medium, and the culture medium in a 500ml Erlenmeyer flask was filled with 100ml, and cultured in a shaker flask at 30°C and 180rpm for 24 hours to obtain liquid strains in the Erlenmeyer flask. Wherein, the seed culture medium is YPD liquid medium, and the composition of YPD liquid medium is: glucose 40g, peptone 10g, yeast powder 10g, 1000ml water, natural pH. The medium was sterilized at 121° C. for 20 minutes at high temperature.
[0035] 2) Preparation of seed tank liquid strain
[0036] The marine rhodotorula triangular flask liquid strain obtained in step 1) was inoculated into the YPD liquid culture medium with 5% inoculum to multiply, and fermented for 24 hours at 28° C. to obtain the liquid strain o...
Embodiment 2
[0044] As an embodiment of the present invention, the preparation method of yeast culture comprises the following steps:
[0045] 1) Preparation of liquid strains in Erlenmeyer flask
[0046] The activated Rhodotorula strains were inoculated into the seed liquid culture medium, and the culture medium in a 500ml Erlenmeyer flask was filled with 100ml, and cultured in a shaker flask at 30°C and 180rpm for 24 hours to obtain liquid strains in the Erlenmeyer flask. Wherein, the seed culture medium is YPD liquid medium, and the composition of YPD liquid medium is: glucose 40g, peptone 10g, yeast powder 10g, 1000ml water, natural pH. The medium was sterilized at 121° C. for 20 minutes at high temperature.
[0047] 2) Preparation of seed tank liquid strain
[0048] The marine rhodotorula triangular flask liquid strain obtained in step 1) was inoculated into the YPD liquid culture medium with 5% inoculum to multiply, and fermented for 24 hours at 28° C. to obtain the liquid strain o...
Embodiment 3
[0056] As an embodiment of the present invention, the preparation method of yeast culture comprises the following steps:
[0057] 1) Preparation of liquid strains in Erlenmeyer flask
[0058] The activated Rhodotorula strains were inoculated into the seed liquid culture medium, and the culture medium in a 500ml Erlenmeyer flask was filled with 100ml, and cultured in a shaker flask at 30°C and 180rpm for 24 hours to obtain liquid strains in the Erlenmeyer flask. Wherein, the seed culture medium is YPD liquid medium, and the composition of YPD liquid medium is: glucose 40g, peptone 10g, yeast powder 10g, 1000ml water, natural pH. The medium was sterilized at 121° C. for 20 minutes at high temperature.
[0059] 2) Preparation of seed tank liquid strain
[0060] The marine rhodotorula triangular flask liquid strain obtained in step 1) was inoculated into the YPD liquid culture medium with 5% inoculum to multiply, and fermented for 24 hours at 28° C. to obtain the liquid strain o...
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