A lamprey immune protein lip mutant that can be used as a tumor diagnostic marker

Abstract

The invention discloses a lamprey immune protein LIP mutant that can be used as a tumor marker, which is characterized in that the amino acid sequence is as shown in SEQ ID NO:1. The asparagine at the 286th amino acid in the lamprey immune protein LIP amino acid sequence is mutated to alanine, which still retains the unique property of being specifically positioned on tumor cells, but greatly reduces the killing function of tumor cells , to avoid destroying the shape of the tumor and affecting the binding force with tumor cells, it can be used as a tumor diagnostic marker to effectively improve the accuracy of tumor diagnosis.

Description

technical field [0001] The invention relates to a tumor diagnostic marker, in particular to a lamprey immune protein LIP mutant which can be used as a tumor diagnostic marker. Background technique [0002] Lamprey immune protein LIP (lamprey plum protein), its cDNA length is 1120bp, encoding 313 amino acids, protein molecular weight is 34.2kD, is a secreted protein. The lamprey immune protein LIP has two functional domains, namely the N-terminal Jacalin-like domain and the C-terminal Aerolysin domain, which can recognize the N-glycan chains of tumor cell GPI-APs and the sheath of tumor cell lipid raft microdomains. Phospholipids localize the lamprey immune protein LIP on the surface of tumor cells; under the action of a small amount of lamprey immune protein LIP, tumor cells will bubble and leak their contents in a short period of time, eventually leading to cell death, and Lamprey immune protein (LIP) has no killing effect on normal cells. Because lamprey immune protein (...

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Problems solved by technology

However, because of its strong killing activity against tumor cells, it destroys the tumor morphology, affects the binding force with tumor cells, and reduces the accuracy of tumor diagnosis.

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