Tissue culture breeding method of stephania epigaea high in cycleanine and cepharanthine content

Abstract

The invention provides a tissue culture breeding method of stephania epigaea high in cycleanine and cepharanthine content. The method includes the following steps that after being selected and sterilized, stephania epigaea explants are inoculated to an axillary bud induction culture medium, and obtained axillary buds are cut off and transferred to a culture medium for bud callus induction; then, bud calluses are transferred to an adventitious bud culture medium to induce differentiation of adventitious buds, and the number of the adventitious buds reaches 10-20 within 40 days; ancymidol is added to inhibit elongation of the buds and induce rooting, and after seedling hardening, transplanting is carried out, wherein the survival rate reaches 90% or above. The method is suitable for industrial production and has the advantages of being high in breeding speed and large in amplification factor, maintaining excellent characters of original varieties and the like.

Description

technical field [0001] The invention relates to a method for tissue culture and breeding of takaninin and stephenin which are incompatible with each other, and belongs to the field of plants. [0002] technical background [0003] Stephania epigaea is a subgenus of stephania (Stephania) in the family Menispermaceae. This genus has huge roots and is exposed on the ground and looks like a turtle. Therefore, it is also called "mountain turtle". It is a traditional Chinese herbal medicine in my country. , its root tubers are used as medicine, and are widely used in various places south of the Yangtze River Basin, especially among ethnic minorities in South China and Southwest China. [0004] Dirong is rich in alkaloids such as stephenin and chrysanthemum, the former is used in the research of leukocyte hyperplasia stephenin tablets, and the latter is the precursor of chrysanthemum (dibromomethane salt of chrysanthemum). Through the investigation of field intolerant resources in w...

AI Technical Summary

Problems solved by technology

The inventor found in the experimental study that the tissue culture and rapid propagation in a place that is not suitable for bud proliferation, the concentration of the hormone BA is 1.0-3.0 mg·L -1 When it is used, it is biased towards the formation of callus, and the effect on the proliferation of buds is not good. Transfer the callus with buds to MS+BA 0.4~0.8mg·L -1 +NAA 0.2mg·L -1 Differentiation of adventitious buds on the upper part of the callus, but it is difficult to differentiate adventitious buds from the callus of the excised upper part of the callus, which requires a longer period of induction and the number is very small. The reason may be that the upper part of the callus synthesizes some trace substances to promote the differentiation of adventitious buds , but the use of the rooting medium provided in the literature can not make the rooting of the rooting of the content of Takanenin and stephenin, directly resulting in the inability to obtain viable grounding tissue

[0006] To sum up, there are technical problems such as low multiplication coefficient, difficulty in inducing adventitious buds, and difficult rooting in tissue culture rapid propagation of takanin and stephenin content, which hinder the rapid propagation of ground tolerance.