Tissue culture breeding method of stephania epigaea high in cycleanine and cepharanthine content

A technology of Lunhuan Teng Ning and Qianjin Pherenin, which is applied in the field of plants, can solve the problems of difficulty in rooting, intolerant of rapid reproduction, and obstacles, and achieve the effects of neat and consistent offspring, easy transplanting and survival, and large reproduction coefficient.

Inactive Publication Date: 2019-07-05
YUNNAN BIOVALLEY PHARMA CO LTD
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor found in the experimental study that the tissue culture and rapid propagation in a place that is not suitable for bud proliferation, the concentration of the hormone BA is 1.0-3.0 mg·L -1 When it is used, it is biased towards the formation of callus, and the effect on the proliferation of buds is not good. Transfer the callus with buds to MS+BA 0.4~0.8mg·L -1 +NAA 0.2mg·L -1 Differentiation of adventitious buds on the upper part of the callus, but it is difficult to differentiate adventitious buds from the callus of the excised upper part of the callus, which requires a longer period of induction and the number is ver

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Material: The ground does not allow female plants to have jointed stems

[0044] 2. Experimental equipment: ultra-clean table, nutrition bag 10×10cm (diameter×height), beaker (250ml), glass rod, timer, tweezers, scissors.

[0045] 3. Method: Using the nodular stem section of the infeminal plant rich in Cynophyllum chinensis and Stephania chinensis as explants, through bud induction, callus induction, adventitious bud induction, rooting, seedling hardening, and transplanting to realize tissue culture and breeding .

[0046] 4. Operation steps:

[0047] 1) Bud induction, select the jointed young stems of female plants as explants, and undergo routine sterilization (washing with running water for 30 minutes, soaking in 75% alcohol for 20-30s, 0.2% HgCl 2 Sterilize for 6-10 minutes, rinse with sterile water for 3-4 times, blot dry with sterile filter paper), inoculate into bud induction medium 1 / 2MS+BA0.5mg·L -1 +IBA0.1mg·L -1 superior;

[0048] 2) Callus induction,...

Embodiment 2

[0055] 1. Material: the segmented stem of the male plant in the ground

[0056] 2. Experimental equipment: ultra-clean table, nutrition bag 10×10cm (diameter×height), beaker (250ml), glass rod, timer, tweezers, scissors.

[0057] 3. Method: The jointed stems of the male plants in the ground are used as explants, and the tissue culture is realized through bud induction, callus induction, adventitious bud induction, rooting, seedling hardening, and transplanting.

[0058] 4. Operation steps:

[0059] 1) Bud induction, select the tender stem section of the male plant with joints as explants, and undergo routine sterilization (washing with running water for 30 minutes, soaking in 75% alcohol for 20-30s, 0.2% HgCl 2 Sterilize for 6-10 minutes, rinse with sterile water for 3-4 times, blot dry with sterile filter paper), inoculate into bud induction medium 1 / 2MS+BA0.2mg·L -1 +IBA0.1mg·L -1 superior;

[0060] 2) Callus induction, cut and transfer to callus induction medium MS+BA 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a tissue culture breeding method of stephania epigaea high in cycleanine and cepharanthine content. The method includes the following steps that after being selected and sterilized, stephania epigaea explants are inoculated to an axillary bud induction culture medium, and obtained axillary buds are cut off and transferred to a culture medium for bud callus induction; then, bud calluses are transferred to an adventitious bud culture medium to induce differentiation of adventitious buds, and the number of the adventitious buds reaches 10-20 within 40 days; ancymidol is added to inhibit elongation of the buds and induce rooting, and after seedling hardening, transplanting is carried out, wherein the survival rate reaches 90% or above. The method is suitable for industrial production and has the advantages of being high in breeding speed and large in amplification factor, maintaining excellent characters of original varieties and the like.

Description

technical field [0001] The invention relates to a method for tissue culture and breeding of takaninin and stephenin which are incompatible with each other, and belongs to the field of plants. [0002] technical background [0003] Stephania epigaea is a subgenus of stephania (Stephania) in the family Menispermaceae. This genus has huge roots and is exposed on the ground and looks like a turtle. Therefore, it is also called "mountain turtle". It is a traditional Chinese herbal medicine in my country. , its root tubers are used as medicine, and are widely used in various places south of the Yangtze River Basin, especially among ethnic minorities in South China and Southwest China. [0004] Dirong is rich in alkaloids such as stephenin and chrysanthemum, the former is used in the research of leukocyte hyperplasia stephenin tablets, and the latter is the precursor of chrysanthemum (dibromomethane salt of chrysanthemum). Through the investigation of field intolerant resources in w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 林艳和杜江李村富林春刘正杰毛自朝
Owner YUNNAN BIOVALLEY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap