A kind of aspergillus fungal strain and application thereof
A technology of Aspergillus fungi and strains, applied in the fields of application, fungi, biocides, etc., can solve 3R problems, achieve simple operation process and good control effect
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Embodiment 1
[0065] ——Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) Acquisition, identification and preservation of ANHHYSFJ01.
[0066] (1) Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) Acquisition and identification of ANHHYSFJ01.
[0067] The fungus of the genus Aspergillus described in the present invention is isolated from red fire ants, which are collected from Dongsanhuan Road, Kunming City, Yunnan Province, Panlong District, Chenggong District, Dongchuan District, Mangshi, Dehong Dai and Jingpo Autonomous Prefecture. The complete nests with RIFA queens were reared in the laboratory.
[0068] Wash the red imported fire ants with a 75% ethanol solution for 5 seconds, then disinfect the surface of the ants with a 0.1% mercury solution for 2-3 minutes, slowly rinse with sterile water for 3 times, and use sterilized The tweezers separate the body of the RIFA according to different parts of the head, thorax and abdomen, and carefully place the different b...
Embodiment 2
[0080] ——Jifeng Aspergillus (Aspergillus genus) Aspergillus nomius ) ANHHYSFJ01 to prepare biocontrol agents.
[0081] (1) Preparation of spore suspension.
[0082] First, the Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) ANHHYSFJ01 strain was inoculated on ordinary PDA medium, and cultured in a 25°C ± incubator for 15 days at a constant temperature.
[0083] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.
[0084] Sterilized sterile water was then added with 0.05% Tween-80.
[0085] Rinse the spores from the Petri dish with sterile aqueous solution.
[0086] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.
[0087] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.
[0088] Finally ...
Embodiment 3
[0092] ——Jifeng Aspergillus (Aspergillus genus) Aspergillus nomius ) ANHHYSFJ01 to prepare biocontrol agents.
[0093] (1) Preparation of spore suspension.
[0094] First, the Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) ANHHYSFJ01 strain was inoculated on ordinary PDA medium and cultured in a 26°C incubator for 15 days.
[0095] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.
[0096] Sterilized sterile water was then added with 0.05% Tween-80.
[0097] Rinse the spores from the Petri dish with sterile aqueous solution.
[0098] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.
[0099] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.
[0100] Finally calculate the total spore numbe...
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