52 results about "Aspergillus aculeatinus" patented technology

The invention belongs to the field of detection of harmful biological molecules in food, and relates to a preparation method and application of an Aspergillus flavus specific single-chain antibody. The cell wall proteins of the Aspergillus flavus hypha are used for immunizing chickens and mice respectively; messenger RNA of chicken splenic lymphocytes after immunization are extracted; a chicken source single-chain antibody library is constructed; a phage display technology is employed to screen the library to obtain an Aspergillus specific single-chain antibody gene with high affinity to Aspergillus flavus. The antibody gene is subcloned into an expression vector of an alkaline phosphatase protein, and expressed and purified in Escherichia coli, so as to obtain a fusion protein and a hybridoma cell 2A8 secreting Aspergillus flavus specific monoclonal antibody. The monoclonal antibody as a capture antibody and AfSA4-AP fusion protein as a detection antibody are employed to establish a SandWich ELISA immunological detection system for detecting Aspergillus flavus contamination in crops and stored plant-derived products.

The invention discloses a method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori 1042-2 which belongs to the Aspergillus and has the collection registration number of M209142 in the China Center for Type Culture Collection (CCTCC). The method for producing the cellulose and the polypeptide protein feed comprises the following steps: A, selecting the Aspergillus awamori 1042-2; B, authenticating the Aspergillus awamori 1042-2, C, culturing the Aspergillus awamori 1042-2 liquid; D, fermenting the solid material; and F, postprocessing. The solid culture which is obtained by inoculating the Aspergillus awamori 1042-2 onto the solid culture medium and fermenting the Aspergillus awamori 1042-2 and the solid culture medium is baked to dry, ground, sieved and subjected to the detection of the enzyme activity and the polypeptide protein content. The method for producing the cellulose and the polypeptide protein feed is feasible and easy, the produced powdered cellulose has the CMC enzyme activity of 31397.1-41826.7 U / g and the FPA enzyme activity of 1779.3-2212.1 U / g, has good stability and can be produced on a large scale, and the produced polypeptide protein feed contains more than 35 percent of protein. The Aspergillus awamori 1042-2 can be used for fermenting the palm kernel dregs, other dregs and the vegetable fiber and producing the biological feed, the biological fertilizer, the biological fuel and can be also used in the food industry, chemical industry and the pharmaceutical industry. The polypeptide protein feed produced by fermenting with the Aspergillus awamori 1042-2 can be used as the additive of the protein of various feed.

The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.

The invention provides compositions comprising an alkene epoxidase and a selective epoxide hydrolase, such as a recombinant microorganism comprising a first heterologous nucleic acid encoding an alkene epoxidase and a second heterologous nucleic acid encoding a selective epoxide hydrolase. Exemplary alkene epoxidases include StyAB, while exemplary selective epoxide hydrolases include epoxide hydrolases from Sphingomonas, Solanum tuberosum, or Aspergillus. The invention also provides non-toxic methods of making enantiomerically pure vicinal diols or enantiomerically pure alpha-hydroxy carboxylic acids using these compositions and microorganisms.

The invention belongs to the technical field of microorganisms, and relates to marine-derived Aspergillus terreus M7 with an antibacterial effect, and separation and application of a secondary metabolite of the Aspergillus terreus M7. The strain is classified and named as Aspergillus terreus M7, and is preserved in China Center for Type Culture Collection (CCTCC) on June 7, 2021, with the preservation number of CCTCC NO: M 2021679. The strain is fermented by a soybean solid culture medium, and is subjected to methanol extraction, silica gel G chromatography, Sephadex LH-20 gel column chromatography, silica gel H chromatography and preparative liquid phase separation and purification to obtain a compound terramide A. The terramide A has obvious antibacterial activity on acinetobacter baumannii ATCC 19606, and has the potential of being developed into an antibacterial agent.

The present invention provides monoclonal antibodies specific for members of the Aspergillus genus and uses thereof for increasing the survival rate of a subject infected with an Aspergillus species. The present invention also provides conjugates of alliinase with monoclonal antibodies specific for members of the Aspergillus genus that preserve the ability of the antibody to recognize the fungus as well as the activity of the enzyme to produce cytotoxic molecules of allicin from the substrate alliin. The invention further provides pharmaceutical compositions comprising the conjugates of the mAbs of the invention and alliinase and uses thereof in combination with the substrate alliin in the treatment of diseases cause by Aspergillus species, in particular invasive aspergillosis.

A composition for degradation of mycotoxin includes Aspergillus culture filtrate as an effective component and uses thereof, and it is expected that, in the field of food products and animal feeds for which biodegradation of mycotoxin (in particular, aflatoxin) is required, and the composition can be advantageously used as a novel material that can maintain the activity of degrading mycotoxin even at high temperatures.

The invention provides a soil phosphorus activator, and belongs to the technical field of biological fertilizers. The soil phosphorus activator comprises, by mass, 0.01-0.1 part of group B metal oxide, 25-60 parts of aspergilli, 30-50 parts of Arbuscular mycorrhizal fungi, 8-12 parts of humic acid and 10-20 parts of bean crop straws; and the effective number of viable bacteria of the aspergilli inthe soil phosphorus activator is equal to or more than 1 * 10<8> CFU / g, and the effective number of spores of Arbuscular mycorrhizal fungi is equal to or more than 15 / g. The soil phosphorus activatorprovided by the invention can activate the phosphorus in wheat field soil, reduce the solidification of phosphorus to unavailable phosphorus, stimulate the absorption of the surrounding phosphorus bywheat roots, increase the utilization rate of a phosphorus fertilizer, reduce the application of the phosphate fertilizer and ensure the stable yield and the yield increase of wheat.

An Aspergillus mutant strain characterized in that it is an auxotrophic mutant strain of Aspergillus oryzae strain AOK27L.

The purpose of the present invention is to provide a technique to duplicate and translocate any large region in a chromosome of a fungus belonging to Aspergillus across a wide range, so that it will possible to stably and systematically acquire an Aspergillus strain having a novel trait, which was un-acquirable by conventional techniques.The present invention relates to a transformant of the fungus belonging to Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5′ or 3′ end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3′ or 5′ end of its coding region is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.

The invention relates to an Aspergillus fungus (Aspergillus sp.EGF15-0-3) and an application thereof. The Aspergillus fungus is preserved at the Guangdong Provincial Microbial Culture Collection Center, and a preservation number is GDMCC 60476. The Aspergillus fungus (Aspergillus sp.EGF15-0-3) of the invention can be used to prepare benzaldehyde ylide heterodimer compounds, a renewable biologicalsource is provided for rare natural products, and thereby a sufficient material basis is provided for further biological activity researches and biological applications of the rare natural products.

The invention provides a compound as well as a synthetic gene cluster and an application thereof. The compound is prepared from a precursor compound aspergidienes A-D and a sesterterpene compound aspergiols A-D, and the aspergiols A-D has the anti-tumor activity, and the sesterterpene compound aspergiols A-D has the anti-tumor activity. The biosynthetic gene cluster SAC11189 composed of a terpenesynthase gene Au11189 and a cytochrome P450 enzyme gene Au11188 is obtained in aspergillus fungi 094102 through genome mining. An aspergillus oryzae expression system is used for expressing each genein a gene cluster to obtain four sesquiterpene compounds aspergiols A-D with novel structures. In-vitro cytotoxic activity test results show that the assergils A-D shows different degrees of inhibitory activity on a plurality of tumor cells, and a possible candidate compound is provided for the development of novel anti-tumor drugs.

The invention provides ATP(CTP): tRNA nucleotidyltransferase (CCA1) as a novel antifungal target, screening methods for CCA1 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine. Specifically in the treatment of an individual susceptible to or suffering from an anti-fungal infection. In particular the compounds find use in the treatment of topical or mucosal (e.g. thrush and vaginal candidiasis) fungal infections, e.g. caused by fungus of the Candida species, and for systemic infections, e.g. caused by fungi of Candida andAspergillus species, such as but not limited to C. Albicans, Aspergillus flavus or Aspergillus fumigatus.

The invention discloses a fungal strain of the genus Aspergillus and its application, and the fungal strain of the genus Aspergillus is classified as Aspergillus nomius , Deposit unit: General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC), deposit date: December 28, 2018, deposit number: CGMCC No.17066, the fungus of the genus Aspergillus belongs to the Kingdom Mycophyta, Phylum Fungi, Silk Sporina, Hyphospora, Leucospora, Aspergillus. The application is the application of the Aspergillus fungus strain in the preparation of biological preparations for biological control of red imported fire ants.

The invnetion provides aspergillus fumigatus strain TMS-26 for producing taxol. The strain can produce the taxol under the condition of liquid fermentation. The strain is collected in China Center For Type Culture Collection (Wuhan University) on June 15, 2014, and the collection number is CCTCC M2014258. The taxol yield of the strain under the conditions of PDA liquid culture medium, 28 DEG C, 60r / min and dark culture for 10 to 12d can reach 696.24[mu]g / L to the maximum degree. The novel strain provided by the invention provides good study basis for the breeding of good strains in industrial large-scale production, and the application prospects are wide.

The invention relates to an Epicoccum purpurascens and Aspergillus spp. coculture method. The coculture method allows aspergillus to generate secondary metabolites which are lowly yielded or not generated in the single culture process. The method comprises the following steps: 1, respectively inoculating an activation medium with Epicoccum purpurascens and Aspergillus spp. to activate strains; 2, inoculating a solid medium in a same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out stationary culture; or inoculating a liquid medium in the same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out shaking culture; and 3, collecting the Aspergillus spp. and the media when Aspergillus spp. spores in the solid medium become yellow in order to obtain a product. The invention also relates to secondary metabolite produced through the coculture method.

The invention belongs to the field of microorganisms, and relates to a method for screening microorganisms capable of producing amylase from soil and carrying out strain identification. A hydrolysis ring is generated by an iodine staining method for preliminary screening, and the enzymatic activity is determined by a 3, 5-dinitrosalicylic acid (DNS) colorimetric method, so that a strain with excellent enzyme production capacity is provided for industrial production. The result shows that the 31 screened strains capable of producing the amylase belong to 10 genus, and the 31 screened strains comprise 9 strains of bacillus (Bacillus), 1 strain of paenibacillus (Paenibacillus), 4 strains of streptomyces (Streptomyces), 2 strains of enterobacter (Enterobacter), 4 strains of Aspergillus (Aspergillus), 2 strains of paecilomyces (Simple), 7 strains of Penicillium (Penicillium), 1 strain of Alternaria (Alternaria) and 1 strain of Cladosporidium (Cladosporidium). Wherein the amylase activity of streptomyces, aspergillus flavus and aspergillus niger is relatively high.

The invention provides a soil phosphorus activator, which belongs to the technical field of biological fertilizers. The soil phosphorus activator comprises the following components in parts by mass: 0.01-0.1 parts of B group metal oxides, 25-60 parts of Aspergillus fungi 30 to 50 parts of arbuscular mycorrhizal fungi of the genus Cystis, 8 to 12 parts of humic acid, and 10 to 20 parts of bean crop straw; in the soil phosphorus activator, the effective number of viable bacteria of the fungus of the genus Aspergillus ≥ 1×10 8 CFU / g, the effective number of spores of arbuscular mycorrhizal fungi of the genus Cystis ≥ 15 / g. The soil phosphorus activator provided by the invention can activate phosphorus in the wheat field soil, reduce the solidification of phosphorus to inactive phosphorus, stimulate the absorption of surrounding phosphorus by wheat roots, improve the utilization rate of phosphorus fertilizer, reduce the application of phosphorus fertilizer, and ensure Stable and increased production of wheat.

The invention discloses a high-efficiency paclitaxel-producing fungus Aspergillus aculeatus Tax-6 and its application. The high-efficiency paclitaxel-producing fungus is named Aspergillus aculeatus ( Aspergillus aculeatinus ) Tax‑6, deposited in the China Center for Type Culture Collection, the deposit number is CCTCC M 2016614, the deposit date is November 3, 2016, and the deposit address is Wuhan University, Wuhan, China. The present invention cultivates Tax‑6 through a liquid fermentation process and then extracts paclitaxel in the fermentation broth, the highest content reaches 334.92 µg / L and the culture time only needs 7 days. The present invention utilizes the endophytic fungus of yew bark to ferment paclitaxel to produce paclitaxel, which not only produces paclitaxel, a high-efficiency anticancer drug, but also protects the rare species of yew.

The invention discloses a strain composition and application thereof as well as a preparation method and application of medicated leaven. The strain composition comprises peritoneum fibuligerum and bacillus subtilis. The strain composition is safe and harmless, has protease and amylase production activity, can be symbiotic and can improve the enzyme activity of medicated leaven, and compared with existing aspergillus fungi used for synergistic fermentation, the yeast fungi avoid production of mycotoxin and are safer.

The invention discloses an aromatic butenolide compound, a preparation method thereof, and an application in the preparation of COX-2 inhibitors and antioxidant drugs. The present invention obtains from a strain of Aspergillus fungus Aspergillus terreus Aromatic butenolide compounds with COX‑2 inhibitory activity and antioxidant activity were isolated from the fermentation product of SCAU011 CGMCC No: 19270 (as shown in Figure 1), in the preparation of COX‑2 inhibitors and antioxidant drugs It has a good application prospect.

The invention discloses a strain of Hubei ophiopogon endophytic aspergillus and application of the strain. The strain of the Hubei ophiopogon endophytic aspergillus is an Aspergillus sp. strain EF029,the preservation number is CCTCC No: M2018781, and the strain was deposited on November 12, 2018. The strain of the Hubei ophiopogon endophytic aspergillus provided by the invention can be used for preparing Hubei ophiopogonpolysaccharide, so that pollution to the environment caused by chemical synthesis in an existing method for preparing the Hubei ophiopogonpolysaccharide is avoided, various unfavorable factors in the planting production of Hubei radix ophiopogonis medicinal materials can be avoided, and the strain of the Hubei ophiopogon endophytic aspergillus is an important microorganismfor searching new resources of the Hubei ophiopogon endophytic aspergillus, and has high application value.

The invention relates to a ratio fluorescence biosensor based on gold nanoclusters, a preparation method of the ratio fluorescence biosensor and application of the ratio fluorescence biosensor to detection of aspergillus mycetes. The method comprises the following steps: by taking an aspergillus mould DNA genome as a target, designing an LAMP primer, and adding FITC-coated AuNCs on the basis of determining the specificity of the primer to carry out LAMP reaction. AuNCs protected by BSA is used as a fluorophore, FITC is used as a pH response signal, along with LAMP reaction, a large number of pyrophosphate and hydrogen ions are released, the ratio fluorescence signals of FITC and AuNCs are gradually reduced, and the method can be used for quantitative analysis of aspergillus mould detection.

A novel process for the preparation of L-threo-dihydroxyphenylserine (Droxidopa) is described. It comprises of enantioselective hydrolysis of racemic (DL)-threo-N-acetyl-3-(3,4-methylenedioxyphenyl)-serine using commercially available L-amino acylase from Aspergillus sp. (EC 3.5.1.14) in the presence of cobalt ions, to obtain (L)-threo-3-(3,4-methylenedioxyphenyl)-serine followed by dealkylation to obtain Droxidopa. Protecting the amino group of (L)-threo-3-(3,4-methylenedioxyphenyl)-serine using either benzyloxycarbonyl or phthaloyl group before dealkylation followed by deprotection of the amino group results in obtaining Droxidopa in high yields and purity.

The invention discloses an endophytic Aspergillus strain of Ophiopogon japonicus Hubei and its application. The endophytic Aspergillus strain of Ophiopogon japonicus Hubei is Aspergillus sp. EF029, the preservation number is CCTCC No: M2018781, and the preservation time is for November 12, 2018. The endophytic Aspergillus strain of Ophiopogon japonicus provided by the present invention can be applied to the preparation of Ophiopogon japonicus polysaccharides, thereby avoiding environmental pollution caused by chemical synthesis in the existing method for preparing Ophiopogon japonicus hubei polysaccharides, and more avoiding the cultivation of Ophiopogon japonicus medicinal materials Various unfavorable factors in the production are important microorganisms to search for new resources of Ophiopogon japonicus polysaccharides in Hubei, and have great application value.

The invention discloses a complex vitamin D for preventing and treating fungal infection. The complex vitamin D comprises functional components of vitamin D2 and a vitamin D3 in the mass ratio of thevitamin D2 to the vitamin D3 being (1-3) to (1-5). The invention further discloses an application of the complex vitamin D to preparation of a medicine for preventing and treating fungal infection. The complex vitamin D has the effects of preventing and treating infection of aspergillus, cryptococcus neoformans and the like. The compound is low in cytotoxicity and can be used as a candidate for treatment of clinical anti-fungal infection.

A seed koji (Aspergillus starter) for brewing is provided. The seed koji is obtained by inoculating koji mold(s) belonging to an Aspergillus genus into a seed koji raw material, where the seed koji raw material is a pea.