Method for separating amylase-producing microorganisms from soil

A technology of amylase and microorganisms, applied in the field of microorganisms, can solve problems such as lack

Pending Publication Date: 2022-06-21
LINYI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of strains with superior performance and higher biological activity of enzymes to meet the needs of industrial production.

Method used

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  • Method for separating amylase-producing microorganisms from soil
  • Method for separating amylase-producing microorganisms from soil
  • Method for separating amylase-producing microorganisms from soil

Examples

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[0035] 1.1 Materials

[0036] 1.1.1 Sample Collection

[0037] The soil was taken from the surface 5-10 cm deep of the green space of the Linyi University campus.

[0038] 1.1.2 Main reagents

[0039] 3,5-Dinitrosalicylic acid: Shanghai McLean Biochemical Technology Co., Ltd.;

[0040] Phenol: Tianjin Damao Chemical Reagent Factory;

[0041] Yeast powder, peptone: OXOID company;

[0042] Sodium sulfite, magnesium sulfate, dipotassium hydrogen phosphate: Ron's reagent;

[0043] Manganese sulfate, ammonium sulfate: Tianjin Bodi Chemical Co., Ltd.;

[0044] Soluble starch, sodium hydroxide, agar powder: Tianjin Hengxing Chemical Reagent Manufacturing Co., Ltd.;

[0045] Potassium sodium tartrate, sodium chloride, calcium chloride, disodium hydrogen phosphate: Tianjin Guangfu Technology Development Co., Ltd.; Iodine, potassium iodide: Shandong Yindian Chemical Co., Ltd.

[0046] 1.1.3 Culture medium

[0047] Solid screening medium: ammonium sulfate 10g / L, sodium chloride 5g / ...

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Abstract

The invention belongs to the field of microorganisms, and relates to a method for screening microorganisms capable of producing amylase from soil and carrying out strain identification. A hydrolysis ring is generated by an iodine staining method for preliminary screening, and the enzymatic activity is determined by a 3, 5-dinitrosalicylic acid (DNS) colorimetric method, so that a strain with excellent enzyme production capacity is provided for industrial production. The result shows that the 31 screened strains capable of producing the amylase belong to 10 genus, and the 31 screened strains comprise 9 strains of bacillus (Bacillus), 1 strain of paenibacillus (Paenibacillus), 4 strains of streptomyces (Streptomyces), 2 strains of enterobacter (Enterobacter), 4 strains of Aspergillus (Aspergillus), 2 strains of paecilomyces (Simple), 7 strains of Penicillium (Penicillium), 1 strain of Alternaria (Alternaria) and 1 strain of Cladosporidium (Cladosporidium). Wherein the amylase activity of streptomyces, aspergillus flavus and aspergillus niger is relatively high.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for separating amylase-producing microorganisms from soil. Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Starch is a polysaccharide naturally stored in higher plants and is a homopolymer formed by the polymerization of glucose molecules. It has good biodegradability and renewability. Starch products are not only still an indispensable basic food source for human beings today, but also provide a large number of important raw materials for modern industry and production activities. Glucose, maltodextrin and maltose are produced by the disosaccharificat...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/20C12N1/14C12R1/11C12R1/01C12R1/465C12R1/685C12R1/67C12R1/80C12R1/82C12R1/645
CPCC12N1/02C12N1/20C12N1/14
Inventor 高翠娟刘晶晶李军奇李德龙
Owner LINYI UNIVERSITY
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