Aspergillus fungus and application thereof

A technology of Aspergillus fungi and strains, applied in the direction of fungi, microorganisms, microorganisms, etc., can solve the problems of low efficiency of extraction methods, unknown synthesis reaction conditions, and high cost

Active Publication Date: 2020-05-12
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of benzaldehyde ylide heterodimer compounds is complex, and the synthesis reaction conditions are unknown, and the yield is low
The reported extraction methods have low efficiency and high cost, so it is of great significance to find new preparation methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aspergillus fungus and application thereof
  • Aspergillus fungus and application thereof
  • Aspergillus fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The separation and purification of embodiment 1 bacterial strain

[0054] Sample source: Soft corals were collected from the waters near Dongluo Island, Sanya, South China Sea.

[0055] (1) Treatment of soft coral samples: After the collected coral samples were treated with sterile seawater, they were placed in a sterile bag, sealed and stored in ice cubes, and the microorganisms were separated as soon as possible.

[0056] (2) Acquisition of symbiotic microorganisms of soft corals: wash the surface of coral samples 3-5 times with a small amount of sterile seawater in an ultra-clean workbench to obtain a solution containing symbiotic microorganisms of corals.

[0057] (3) Acquisition of endophytic microorganisms in soft corals: wipe the surface of the above-mentioned coral samples rinsed with sterile seawater with sterile alcohol to kill surface microorganisms and prevent contamination; cut open the corals with a scalpel, get their internal tissues, and use a mortar A ...

Embodiment 2

[0068] Example 2 Preparation of Cryptoechinulin D by Aspergillus EGF15-0-3

[0069] (1) Strain activation: use an inoculation loop to get an appropriate amount of seed solution from the Aspergillus EGF15-0-3 strain preservation tube, inoculate into 50ml strain culture medium (potato 200g / L, glucose 2.0%, potato juice Prepare with old seawater as solvent, in 100ml Erlenmeyer flask of natural pH), culture in 165r / min shaker at 28 ℃ for 2 days, obtain the seed culture solution of Aspergillus EGF15-0-3.

[0070] (2) Fermentation: Draw 1.5ml of EGF15-0-3 seed culture solution and add 400ml of glutinous rice solid medium (glutinous rice 45~55g, aged seawater 80~100ml, peptone 0.25~0.35%) in a 1000ml Erlenmeyer flask, at 28°C Static culture for 60 days, compared with the blank medium to observe whether there is obvious strain growth, the strain growth diagram is as follows Figure 4 As shown (A is the blank medium, B is the medium for the growth of the strain), after the growth is c...

Embodiment 3

[0073] Example 3 Preparation of Cryptoechinulin D by Aspergillus EGF15-0-3

[0074] (1) Activation of strains: consistent with Example 2.

[0075] (2) Fermentation: absorb 1.5ml of EGF15-0-3 seed solution and add 400ml of corn solid medium (50g of northeast rice, 5g of corn dregs, 80ml of old seawater, 0.3% peptone) into a 1000ml Erlenmeyer flask, and let stand at 28°C Cultivate for 60 days, compare with the blank culture medium to observe whether there is obvious strain growth, the strain growth diagram is as follows: Figure 5 As shown (A is the blank medium, B is the medium for the growth of the strain), after the growth is completed, 5-10ml of ethyl acetate is added to stop the fermentation. 100L of co-fermentation solid medium for separation experiments.

[0076] (3) Extraction: The extraction process was consistent with Example 2 to obtain an extract (E1, 80 g).

[0077] (4) Separation: Consistent with Example 2, Cryptoechinulin D (1) (160-280 mg) was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to an Aspergillus fungus (Aspergillus sp.EGF15-0-3) and an application thereof. The Aspergillus fungus is preserved at the Guangdong Provincial Microbial Culture Collection Center, and a preservation number is GDMCC 60476. The Aspergillus fungus (Aspergillus sp.EGF15-0-3) of the invention can be used to prepare benzaldehyde ylide heterodimer compounds, a renewable biologicalsource is provided for rare natural products, and thereby a sufficient material basis is provided for further biological activity researches and biological applications of the rare natural products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to fungi of the genus Aspergillus and applications thereof. Background technique [0002] The structural characteristics of ylide alkaloids are diketopiperazine compounds formed by two molecules of amino acids. In such substances, if one molecule of amino acid is tryptophan, it constitutes common indole diketopiperazine alkaloid derivatives (Borthwick AD, Chem. Rev. 2012, 112, 3641-3176). The isopentenyl indole diketopiperazine alkaloids are formed by indole diketopiperazine compounds through the migration reaction of isopentenyl under the catalysis of indole prenyltransferase, and widely exist in Fungi Aspergillus sp., Penicillium sp., Pestalotiopsis sp. and Chromocleistasp. (Li SM, Natural Products Reports., 2010, 27, 57-78). Compared with non-prenyl substituted indole diketopiperazine alkaloids, most of these prenyl substituted indole diketopiperazine alkaloids have a wide range of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/16C12P17/18C12R1/66
CPCC12P17/165C12P17/162C12P17/18C12R2001/66C12N1/145
Inventor 张翠仙韦霞冯婵刘炳新张琪
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap