Plant protoplast culturing method

Abstract

The invention discloses a plant protoplast culturing method. According to the plant protoplast culturing method, in a suspension culture condition, compound sodium nitrophenolate is employed to activate protoplasm and reinforce the intensity of stimulation to the protoplasm, so that in-vitro amplification capacity of the protoplasm is improved; a high-temperature water bath and then a quick ice bath are performed before elution of the protoplasm, so that a low yield rate caused by adhesion of the protoplasm is avoided. The method is easily controllable in culture process, simple and convenientto operate and easy for expansion.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a plant protoplast culture method. Background technique [0002] Plant protoplast fusion technology is based on the research results of animal cell fusion and established on the basis of protoplast isolation and culture. Using plant protoplasts as materials, the two protoplasts are fused through the induction of physical and chemical factors. together so as to form fused cells. It is not a union between male and female spores, but a fusion between somatic cells with complete genetic material, a hybrid between two kinds of protoplasts. Two cells with different genomes can be combined through protoplast fusion. Compared with conventional sexual hybridization, it can not only expand the range of hybrid parent combinations, but also shorten the breeding cycle and reduce the risk of breeding new varieties. Small. But so far, plant protoplast fusion breeding is still very difficult, the ...

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Problems solved by technology

But until now, plant protoplast fusion breeding is still very difficult, the main reason is that the regeneration efficiency of protoplasts to form plants is extremely low, the repeatability is poor, and it is also genotype dependent.