Portable AHL molecular imprinting silk-screen printing electrochemical detector

A technology of screen printing electrodes and molecular imprinting, applied in the direction of material electrochemical variables, etc., can solve the problems of large number of molecularly imprinted membranes and waste of resources, and achieve the effects of high specificity, increased sensitivity, and increased electron transfer rate.
CN110006984AActive Publication Date: 2019-07-12JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-07-12

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Abstract

The invention discloses a portable AHL molecular imprinting silk-screen printing electrochemical detector, and belongs to the technical field of rapid detection. According to the invention, the surface of a working electrode is coated with a gold nano cross in a dropping manner; then, an AHL molecular analogue is used as a template; a gold-containing electrode is put into an electropolymerizationsolution containing a monomer, a cross-linking agent, a template and an initiator by adopting a surface imprinting technology for electropolymerization; template molecules are eluted to obtain an AHLmolecular imprinting silk-screen printing electrode, a peak current difference value before and after adsorption is obtained based on the electrode in cooperation with specific electrochemical detection conditions, a standard curve is obtained by using the concentration of a to-be-detected substance and the peak current difference value, and the AHL content is calculated. The detector disclosed bythe invention can be used for directly detecting AHL molecules, has the advantages of sensitivity, rapidness, recycling for multiple times, high specificity and the like, is low in price, and is suitable for detecting the AHL molecules on a base layer or on site.
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Description

technical field

[0001] The invention relates to a portable AHL molecular imprinting screen printing electrochemical detector, which belongs to the technical field of rapid detection. Background technique

[0002] Quorum sensing is a unique and diverse group behavior phenomenon that occurs when bacteria grow to a certain density and interact with each other, and perform gene expression and regulation. N-acyl homoserine lactone (AHL) compounds are the most important signal molecules in quorum sensing of Gram-negative bacteria, regulating the expression of many physiological characteristics genes. Rapid, simple and effective detection of whether bacteria can produce AHL has become an important means for in-depth research and understanding of bacterial quorum sensing.

[0003] The principles of various detection methods for AHL molecules are different, and each method has its own advantages and disadvantages. However, due to the improvement of the sensitivity and accuracy of de...

Claims

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