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Application of platelet microparticles in elevated expression of cell tissue factors

A cell tissue and microparticle technology, applied in the field of biomedicine, can solve the problem of unresearched cell tissue factor decryption, etc., and achieve high specificity.

Inactive Publication Date: 2019-07-30
SHENZHEN HUISONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1967, Wolf first discovered and described platelet particles and their procoagulant activity, but the "decryption" of tissue factor has not been studied

Method used

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  • Application of platelet microparticles in elevated expression of cell tissue factors
  • Application of platelet microparticles in elevated expression of cell tissue factors
  • Application of platelet microparticles in elevated expression of cell tissue factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Experimental scheme

[0034] 1. Preparation of PMPs

[0035] (1) Preparation of platelet-rich plasma (PRP): draw venous blood in the morning fasting resting state, discard the first 2 mL of blood, add 109 mmol / L sodium citrate (1:9) anticoagulant tubes, of which 10 2.7 mL was drawn into a sodium citrate anticoagulant tube and another 2.7 mL was drawn into a CTAD anticoagulant tube from a healthy person undergoing physical examination. Immediately after mixing, centrifuge at 1100 rpm / min for 10 minutes; take the upper platelet-rich plasma; adjust the number of PRP platelets to 3×10 11 / L.

[0036] (2) Platelet activation: Add activator ADP (final concentration 20 μmol / L) and collagen (final concentration 20 μg / L) to 200 μL PRP, incubate at room temperature for 10 min, avoid shaking during incubation.

[0037] (3) Immunofluorescence labeling: take 10 μL of PRP without activator or activated with activator, add 5 μL FITC-CD61 and incubate at room temperature i...

Embodiment 2

[0059] Example 2 Flow Cytometry Detection of "Deciphered" Tissue Factor

[0060] 1. Add an appropriate amount of PMP suspension to leukemia cells and fully stimulate them at a constant temperature (37°C). FCM results show ( figure 2 ), 0.91% of the cells in the undecrypted group expressed TF protein, and the average fluorescence expression rate of the cells was 1.52%; 32.97% of the cells in the PMPs "decryption" group expressed TF protein, and the average fluorescence expression rate of the cells was 32.26%; Many cells expressed TF protein, and the amount of TF protein expressed by a single cell also increased significantly.

[0061] 2. Determination of procoagulant activity and TF expression of leukemia cells after stimulation

[0062] The percentage of cells expressing fluorescence in the two groups and the relative average fluorescence expression rate of the cells after "decryption" are as follows: image 3 shown.

[0063] 3. cTF in various types of leukemia patients

...

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Abstract

The application of platelet microparticles in complications of elevated expression of leukemia cell tissue factors belongs to the field of biomedicine. In particular, the present invention discloses an application of platelet microparticles in preparation of agents for diagnosing or predicting elevated expression of cell tissue factors in a leukemia patient, and an application of an annexin V in preparation or screening of drugs for preventing or treating complications caused by elevated expression of cell tissue factors in a leukemia patient. The invention has an important value for assessment and timely treatment of malignant tumor patient patients, in particular to hematologic tumor patients in critical condition when applied in clinical practices.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of platelet microparticles in increasing the expression of tissue factor in cells. Background technique [0002] Cell tissue factor (cTF) is normally in a closed state, and the activation of cell tissue factor is called cTF "decryption" by many scholars. [0003] Platelet microparticles (platelet microparticles, PMPs) are small particles formed by the stretching and deformation of the cell membrane of platelets under the action of different stimuli. one. Its diameter is less than 0.5 μm, its shape cannot be observed by ordinary microscope, and it cannot be detected by conventional methods of detecting platelets including platelet counter. PMP is smaller than normal platelets but has a complete membrane structure. The platelet particle membrane carries most of the components on the platelet membrane in the resting state, and also carries markers on the activated pl...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68G01N33/533G01N15/14A61K38/17A61P35/02A61P7/04A61P7/02
CPCA61K38/17A61P7/02A61P7/04A61P35/02G01N15/1434G01N33/533G01N33/57426G01N33/6863
Inventor 任华平李洁魏文宁黄银艳范海艳
Owner SHENZHEN HUISONG TECH DEV
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