Separated carp antiviral protein Pdcd6ip and antiviral activity

An anti-virus and carp spring viremia technology, which is applied in the direction of antiviral agents, peptide/protein components, and vector-borne diseases, can solve the problem of lack of effective control of carp spring viremia drugs and methods, etc., to reduce infectious effect

Active Publication Date: 2019-08-06
上海市水产研究所(上海市水产技术推广站)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the internationally accepted effective measures to control the disease are to monitor carp spring viremia, detect

Method used

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  • Separated carp antiviral protein Pdcd6ip and antiviral activity
  • Separated carp antiviral protein Pdcd6ip and antiviral activity
  • Separated carp antiviral protein Pdcd6ip and antiviral activity

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Embodiment Construction

[0033] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0034] (1) RNA extraction from carp

[0035] The total RNA of the samples to be tested was extracted by the classic Trizol method. Cut carp tissue pieces with ophthalmic scissors, put them directly into a mortar, add a small amount of liquid nitrogen, and grind them quickly. ) for 10 minutes, and then centrifuged at 12000×g for 5 minutes at 4°C.

[0036] Add 200 μL of chloroform to the supernatant, vortex and mix well, place at room temperature for 15 min, centrifuge at 12000×g for 15 min at 4 °C.

[0037] Take the upper aqueous phase to another new centrifuge tube, add 500 μL of isopropanol, place...

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Abstract

The invention relates to the technical field of freshwater fish genetic engineering, and discloses a separated carp antiviral protein Pdcd6ip and an antiviral activity. The nucleotide sequence of a carp antiviral protein Pdcd6ip gene is a sequence shown in 1st-2610th bp in SEQ ID NO:1, and an encoded amino acid sequence is a sequence shown in SEQ ID NO:2. Tests indicate that the expression of thecloned Pdcd6ip protein can affect the proliferation of spring viremia of carp virus (SVCV), the Pdcd6ip protein plays an important role in the process of cells resisting to SVCV infection, and at thesame time, the effect on the cell activity of ordinary cells is low. The gene or protein provides a new target for preparing anti-SVCV drugs.

Description

technical field [0001] The invention relates to the technical field of freshwater fish genetic engineering, in particular to an isolated carp antiviral protein Pdcd6ip and its antiviral activity. Background technique [0002] my country is a big aquaculture country, but with the continuous development of aquaculture industry in recent years, the incidence of aquatic animal diseases, especially viral diseases, has shown a clear upward trend. This has caused huge economic losses to my country's aquaculture industry and import and export trade, seriously restricting the healthy and sustainable development of my country's aquaculture industry. Among them, Spring viraemia of carp (SVC) is a fulminant hemorrhagic disease caused by Spring viraemia of carp virus (SVCV). The disease is an acute epidemic disease with hemorrhage as the main clinical symptom and highly contagious. It can cause obvious symptoms in many carps such as carp, koi, grass carp, silver carp, bighead carp, cruc...

Claims

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Application Information

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IPC IPC(8): C07K14/46C12N15/12C12N15/85A61K38/17A61P7/04A61P31/14
CPCC07K14/461C12N15/85A61P7/04A61P31/14A61K38/00Y02A50/30
Inventor 邵玲汤茜肖雨
Owner 上海市水产研究所(上海市水产技术推广站)
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