Markers of miRNAs for diagnosis and treatment of liver cancer

A liver cancer, mir-4326 technology, applied in the field of biomedicine, can solve problems such as short survival and patients losing opportunities for surgery

Active Publication Date: 2020-11-10
THE THIRD HOSPITAL OF HEBEI MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of clear early diagnostic markers and specific clinical symptoms, etc., patients with liver cancer are usually diagnosed at an advanced stage, and most patients lose the opportunity for surgery, and the overall 5-year survival period is very short

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Markers of miRNAs for diagnosis and treatment of liver cancer
  • Markers of miRNAs for diagnosis and treatment of liver cancer
  • Markers of miRNAs for diagnosis and treatment of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 QPCR detection of miR-4326 expression in hepatocellular carcinoma

[0043] 1. Experimental materials

[0044] 1. Experimental reagents and instruments

[0045] miRNA first-strand cDNA synthesis, Sangon, Cat. No. B532451-0020,

[0046] miRNA Fluorescent Quantitative PCR Kit, Sanko, Cat. No. B532461-0002,

[0047] NanoVue Plus, BIOCHROM LTD, model 28956057,

[0048] Fluorescent quantitative PCR instrument, Applied Biosystems, model ABI7300

[0049] 2. Experimental samples

[0050] The cancer tissues and the corresponding paracancerous tissue samples > 3cm were collected from 32 cases of primary liver cancer patients diagnosed by histopathology. All samples were put into cryopreservation tubes within 30 minutes after surgical resection, and quickly put into liquid nitrogen tanks. , and transferred to the laboratory -80°C ultra-low temperature freezer.

[0051] Exclusion criteria: patients who had undergone anti-tumor treatment before operation, and had a hi...

Embodiment 2

[0084] Example 2 Verifying the diagnostic efficacy of biomarkers

[0085] 1. Data collection

[0086] The miRNA expression profile data of 342 liver cancer tissues and 50 paracancerous tissues were collected from the TCGA database, and the expression levels of miR-4326 in liver cancer tissues and paracancerous tissues were analyzed.

[0087] 2. ROC curve analysis

[0088] The receiver operating characteristics of miR-4326 were analyzed using the pROC package in R language, the binomial exact confidence space was calculated, and the ROC curve was drawn.

[0089] 3. Results

[0090] The ROC analysis results of miR-4326 are as follows figure 2 As shown, the AUC value of miR-4326 as the test result variable is 0.743, which has a relatively high area under the curve, indicating that the use of miR-4326 in the diagnosis of liver cancer has high sensitivity and specificity, indicating that the application of miR-4326 to The diagnosis of liver cancer has a good diagnostic efficie...

Embodiment 3

[0091] Example 3 Detection of the expression level of miRNA in liver cancer cells

[0092] 1. Cell culture

[0093] Culture human normal liver cell line L-02, liver cancer cell line HepG2 with low invasion and metastasis ability, and liver cancer cell line Huh7 with high invasion and metastasis ability. Mycin 1640 cell culture medium at 37°C, 5% CO 2 and cultured under conditions of saturated humidity. The cells were replaced every 2 days and passaged at a ratio of 1:3.

[0094] 2. Extraction of cellular RNA

[0095] Total cellular RNA was extracted using the TRIzol method.

[0096] Collect the cells in the logarithmic phase, add 1ml TRIzol, and mix well; draw repeatedly with a 1ml syringe to rupture the cells and cut the DNA, let stand at room temperature for 5min, and the rest of the steps are the same as in Example 1.

[0097] 3. Reverse transcription and fluorescence quantitative detection

[0098] The experimental process is the same as in Example 1.

[0099] 4. St...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a marker of miRNA (micro ribonucleic acid) for diagnosing and treating liver cancer. The invention records that expression of miR-4326 is increased in liver cancer, miR-4326 isverified in TCGA (the cancer genome atlas), and results show that miR-4326 has a good diagnosis effect. The invention also records that a preparation of miR-4326 is capable of degrading hepatoma carcinoma cell proliferation activity and reducing the transmembrane number of cells, and reminds that miR-4326 can be applied to diagnosis and treatment on liver cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to markers of miRNA used for diagnosis and treatment of liver cancer. Background technique [0002] Primary liver cancer (HCC) ranks fourth in the incidence of cancer in my country, and ranks second in human deaths caused by malignant tumors. Due to the lack of clear early diagnostic markers and specific clinical symptoms, patients with liver cancer are usually diagnosed at an advanced stage, and most patients lose the opportunity for surgery, and the overall 5-year survival period is very short. Therefore, it is the current research focus to study the inducing factors and the process of invasion and metastasis of liver cancer, and then to develop drugs for liver cancer treatment. [0003] MicroRNA (miRNA) is a class of highly conserved single-stranded endogenous non-coding RNA small molecule fragments with a length of 21-25nt. The transcription of miRNA is mainly catalyzed by polymerase II...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 赵鑫张颖曹经琳刘文鹏刘宝旺崔自强曾强王洋滕亮张军红高庆军窦剑
Owner THE THIRD HOSPITAL OF HEBEI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap