Application of interleukin 37 and its effect on RNA m6A methylation in lung cancer cells

A technology of lung cancer cells and test methods, which can be used in interleukin, tumor/cancer cells, chemical instruments and methods, etc., and can solve problems such as insufficient research

Active Publication Date: 2021-07-30
QINGDAO CENT HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In summary, the mechanism of IL-37 inhibiting the biological behavior of lung cancer is not sufficient

Method used

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  • Application of interleukin 37 and its effect on RNA m6A methylation in lung cancer cells
  • Application of interleukin 37 and its effect on RNA m6A methylation in lung cancer cells
  • Application of interleukin 37 and its effect on RNA m6A methylation in lung cancer cells

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Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0025] 1.1 Cell culture;

[0026] Human lung adenocarcinoma cell line A549 was purchased from Shanghai Gefan Biotechnology Co., Ltd. Human lung adenocarcinoma cells used DMEM-F12 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin, and human normal lung epithelial cells used 1640 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin, placed at 37°C, 5% CO 2 cultured in an incubator. Inoculate well-grown 1×10^6 A549 cells in a 96mm-diameter petri dish, add 10ml of medium for culture, and when the cell fusion rate is about 50%, replace the medium with 5ml of polybrene mixed medium containing 5μg / ml , add 15 μl of lentiviruses carrying NC and IL-37 plasmids respectively, and then add 5 ml of mixed medium for culture after 4 hours...

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Abstract

The invention discloses the application of interleukin 37 and the test method of its influence on the RNA m6A methylation of lung cancer cells. The present invention starts from epigenetics, and by performing MeRIP-seq (Methylated RNA Immunoprecipitation Sequencing) detection on IL-37-overexpressed lung cancer A549 cells, it is found that IL-37 has a significant impact on the RNA m6A modification of A549 cells, indicating that IL-37 can affect the biological behaviors of lung cancer, such as proliferation and metastasis, by means of RNA m6A.

Description

technical field [0001] The invention relates to the application of interleukin 37 and a test method for its influence on RNA m6A methylation of lung cancer cells, belonging to the field of biochemistry. Background technique [0002] The incidence and mortality of cancer in China are increasing year by year. According to statistics, there were about 4.292 million new cancer cases and 2.814 million cancer deaths in China in 2015. Lung cancer is the most common cancer and the leading cause of cancer death. The number of patients with lung adenocarcinoma accounts for about 80% of all lung cancer patients, and most patients with lung adenocarcinoma are already in the advanced stage when they are diagnosed, and the 5-year survival rate after operation is poor. At present, the treatment methods for lung adenocarcinoma include surgery, radiotherapy and chemotherapy, and targeted therapy. Targeted therapy plays an increasingly important role in cancer treatment because of its precisi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/54C12N5/09C12N15/10C40B50/06G16B5/00
CPCC07K14/54C12N5/0625C12N5/0693C12N15/1003C12N2500/02C12N2501/23C40B50/06G16B5/00
Inventor 王晔陈文朱懿峰王鹏牟晓峰张春玲兰克涛
Owner QINGDAO CENT HOSPITAL
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