Application of interleukin 37 and its effect on RNA m6A methylation in lung cancer cells
A technology of lung cancer cells and test methods, which can be used in interleukin, tumor/cancer cells, chemical instruments and methods, etc., and can solve problems such as insufficient research
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[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.
[0025] 1.1 Cell culture;
[0026] Human lung adenocarcinoma cell line A549 was purchased from Shanghai Gefan Biotechnology Co., Ltd. Human lung adenocarcinoma cells used DMEM-F12 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin, and human normal lung epithelial cells used 1640 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin, placed at 37°C, 5% CO 2 cultured in an incubator. Inoculate well-grown 1×10^6 A549 cells in a 96mm-diameter petri dish, add 10ml of medium for culture, and when the cell fusion rate is about 50%, replace the medium with 5ml of polybrene mixed medium containing 5μg / ml , add 15 μl of lentiviruses carrying NC and IL-37 plasmids respectively, and then add 5 ml of mixed medium for culture after 4 hours...
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